Downregulation of β-catenin and Akt signaling is responsible for poor proliferation of the late passage of bone marrow mesenchymal stem cells

Wu,Junfang; Niu,Jie; Li,Xiaopeng; Ren,Tongming; Zhang,Fenxi

doi:10.3892/mmr.2014.2286

Downregulation of β-catenin and Akt signaling is responsible for poor proliferation of the late passage of bone marrow mesenchymal stem cells

Authors:
- Junfang Wu
- Jie Niu
- Xiaopeng Li
- Tongming Ren
- Fenxi Zhang
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Affiliations: Laboratory of Morphology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Ophthalmology, The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Published online on: May 29, 2014 https://doi.org/10.3892/mmr.2014.2286
Pages: 955-958

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Abstract

It is well established that mesenchymal stem cells (MSCs) will partially lose their proliferative ability with continuous expansion. However, the specific mechanisms underlying this effect remain unclear. In the present study, it was identified that β-catenin was downregulated in the late passage (passage 8) of bone marrow mesenchymal stem cells (bmMSCs). Following β-catenin expression, the expression of phospho-Akt was also significantly decreased in the late passage of bmMSCs. More notably, overexpression of β-catenin in passage 8 of bmMSCs by transfection with pMXs-β-catenin plasmids, significantly increased cell proliferation and Akt expression. These results indicate that the downregulation of β-catenin and Akt signaling may be a critical factor for the proliferation of the late passage of bmMSCs.

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