Expression of latent membrane proteins in Epstein‑Barr virus-transformed lymphocytes in vitro

Tang,Yunlian; Luo,Chunyan; Cheng,Ailan; Lu,Suli; Xu,Jinhua; Fu,Ting; Gan,Runliang

doi:10.3892/mmr.2014.2313

Expression of latent membrane proteins in Epstein‑Barr virus-transformed lymphocytes in vitro

Authors:
- Yunlian Tang
- Chunyan Luo
- Ailan Cheng
- Suli Lu
- Jinhua Xu
- Ting Fu
- Runliang Gan
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Affiliations: Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China
Published online on: June 10, 2014 https://doi.org/10.3892/mmr.2014.2313
Pages: 1117-1121

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Abstract

Infection with Epstein-Barr virus (EBV) induces activation and proliferation of B lymphocytes. Detection of latent membrane protein (LMP)-1 is used to identify the proliferative ability of B cells. However, changes in the expression levels of the three LMPs during EBV-induced B lymphocyte transformation, have not yet been reported. In the present study, the expression levels of LMP-1, LMP-2A and LMP-2B were compared between EBV-transformed B lymphocytes and paired normal lymphocytes. Seven lymphoblast cell lines were established by EBV infection of normal human lymphocytes in vitro. The expression levels of LMP genes and LMP-1 protein were determined using quantitative (q)PCR and western blotting in lymphoblasts and normal lymphocytes, respectively. The expression of LMP1, LMP-2A and LMP-2B genes was significantly upregulated in EBV-induced lymphoblasts compared with the normal lymphocytes. The LMP-1 protein level was also significantly increased in EBV-transformed B lymphocytes. Expression of LMP1, LMP-2A and LMP-2B genes was significantly upregulated in EBV-induced lymphoblasts, suggesting LMP genes are important in the transformation of human lymphocytes.

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