Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells

Luo,Jinghua; Yuan,Yong; Chang,Pengyu; Li,Dawei; Liu,Zhiqiang; Qu,Yaqin

doi:10.3892/mmr.2014.2318

Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells

Authors:
- Jinghua Luo
- Yong Yuan
- Pengyu Chang
- Dawei Li
- Zhiqiang Liu
- Yaqin Qu
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Affiliations: Department of Radiotherapy, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Gynecology, The Tumor Hospital of Jilin, Changchun, Jilin 130021, P.R. China, Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: June 10, 2014 https://doi.org/10.3892/mmr.2014.2318
Pages: 731-736

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Abstract

The aim of the present study was to investigate the effects of aloe-emodin (AE) on the radiosensitivity and differentiation of HeLa human cervical cancer cells. Cell proliferation was assessed in the HeLa cervical cancer cell line by a methylthiazolyldiphenyl-tetrazolium bromide assay. Radiosensitivity was determined by a colony‑forming assay. Flow cytometry was used for analysis of cell cycle distribution and apoptosis. The expression of γ-H2AX and cyclin B was assessed by western blotting. Alkaline phosphatase (ALP) activity was measured by an ALP activity kit. It was demonstrated that AE inhibited the proliferation of HeLa cells in a concentration- and time-dependent manner, induced G2/M and S phase cell cycle arrest and enhanced the radiosensitivity of HeLa cells. The combination of AE and radiation induced apoptosis, upregulated cyclin B and γ-H2AX expression and further improved ALP activity compared with treatment with AE or radiation alone. AE enhanced the radiosensitivity of HeLa human cervical cancer cells in vitro, inhibited the proliferation of HeLa cells, induced G2/M phase cell cycle arrest and, in combination with radiation, induced the apoptosis and improved the differentiation of HeLa cells.

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