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Platelet‑derived growth factor promotes osteoblast proliferation by activating G‑protein‑coupled receptor kinase interactor‑1

  • Authors:
    • You Wu
    • Ying Zhang
    • Qingshui Yin
    • Hong Xia
    • Jiajia Wang

  • View Affiliations

  • Published online on: July 14, 2014     https://doi.org/10.3892/mmr.2014.2374
  • Pages: 1349-1354
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Platelet‑derived growth factor (PDGF) has been reported to stimulate bone fracture‑healing. Multiple studies have demonstrated that PDGF has a critical role in osteoblast or osteoprogenitor cell activation, although the underlying mechanism remains unclear. Studies have found that G‑protein‑coupled receptor kinase interactor‑1 (GIT1) is activated by PDGF and described as an important factor in bone metabolism. In the present study, the effects of PDGF and GIT1 on the proliferation and apoptosis of osteoblasts were investigated in cultured osteoblasts isolated from rat calvaria with PDGF stimulation and GIT1 small interfering RNA transfection. The results demonstrated that PDGF rapidly stimulated GTI1 expression in osteoblasts, increased osteoblast proliferation and inhibited cell apoptosis. Furthermore, cyclin D1 expression was significantly upregulated, the number of cells in the G0/G1 phase was decreased, while the number in the S phase was increased. In cells with knockdown of GIT1, the change stimulated by PDGF was not evident. The results indicate that, PDGF stimulated GIT1 activation of cyclin D1 expression, thereby promoting osteoblasts to enter the S phase from the stationary G0/G1 phase, leading to the proliferation of osteoblasts.
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September-2014
Volume 10 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Wu Y, Zhang Y, Yin Q, Xia H and Wang J: Platelet‑derived growth factor promotes osteoblast proliferation by activating G‑protein‑coupled receptor kinase interactor‑1. Mol Med Rep 10: 1349-1354, 2014.
APA
Wu, Y., Zhang, Y., Yin, Q., Xia, H., & Wang, J. (2014). Platelet‑derived growth factor promotes osteoblast proliferation by activating G‑protein‑coupled receptor kinase interactor‑1. Molecular Medicine Reports, 10, 1349-1354. https://doi.org/10.3892/mmr.2014.2374
MLA
Wu, Y., Zhang, Y., Yin, Q., Xia, H., Wang, J."Platelet‑derived growth factor promotes osteoblast proliferation by activating G‑protein‑coupled receptor kinase interactor‑1". Molecular Medicine Reports 10.3 (2014): 1349-1354.
Chicago
Wu, Y., Zhang, Y., Yin, Q., Xia, H., Wang, J."Platelet‑derived growth factor promotes osteoblast proliferation by activating G‑protein‑coupled receptor kinase interactor‑1". Molecular Medicine Reports 10, no. 3 (2014): 1349-1354. https://doi.org/10.3892/mmr.2014.2374