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Berberine attenuates spontaneous action potentials in sinoatrial node cells and the currents of human HCN4 channels expressed in Xenopus laevis oocytes

  • Authors:
    • Hui Chen
    • Yongjun Chen
    • Yanhong Tang
    • Jing Yang
    • Dandan Wang
    • Taihui Yu
    • Congxin Huang

  • View Affiliations

  • Published online on: July 14, 2014     https://doi.org/10.3892/mmr.2014.2377
  • Pages: 1576-1582
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

The present study investigated the electropharmacological effects of a traditional Chinese herbal drug, berberine, on the spontaneous activity of sinoatrial nodes (SANs) of the rabbit heart and on human hyperpolarization-activated cyclic nucleotide-gated 4 (hHCN4) channels, which are heterologously expressed in xenopus oocytes, and which contribute to pacemaker currents (Ifs). A standard microelectrode technique and standard two‑electrode voltage‑clamp recordings were employed to examine the properties of transmembrane potentials and cloned hHCN4 subunit currents, respectively, under control conditions and berberine administration. Berberine decreased the rate of pacemaker firing and the rate of diastolic depolarization, and modified the action potential parameters. In addition, berberine suppressed the hHCN4 channel currents in a concentration‑ (1‑300 µM) and use‑dependent manner, and simultaneously decreased the activation and deactivation kinetics of the hHCN4 channels. The ability of berberine to modulate the If of cardiac pacemaker cells may contribute to its antiarrhythmic action.
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September-2014
Volume 10 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Chen H, Chen Y, Tang Y, Yang J, Wang D, Yu T and Huang C: Berberine attenuates spontaneous action potentials in sinoatrial node cells and the currents of human HCN4 channels expressed in Xenopus laevis oocytes. Mol Med Rep 10: 1576-1582, 2014.
APA
Chen, H., Chen, Y., Tang, Y., Yang, J., Wang, D., Yu, T., & Huang, C. (2014). Berberine attenuates spontaneous action potentials in sinoatrial node cells and the currents of human HCN4 channels expressed in Xenopus laevis oocytes. Molecular Medicine Reports, 10, 1576-1582. https://doi.org/10.3892/mmr.2014.2377
MLA
Chen, H., Chen, Y., Tang, Y., Yang, J., Wang, D., Yu, T., Huang, C."Berberine attenuates spontaneous action potentials in sinoatrial node cells and the currents of human HCN4 channels expressed in Xenopus laevis oocytes". Molecular Medicine Reports 10.3 (2014): 1576-1582.
Chicago
Chen, H., Chen, Y., Tang, Y., Yang, J., Wang, D., Yu, T., Huang, C."Berberine attenuates spontaneous action potentials in sinoatrial node cells and the currents of human HCN4 channels expressed in Xenopus laevis oocytes". Molecular Medicine Reports 10, no. 3 (2014): 1576-1582. https://doi.org/10.3892/mmr.2014.2377