Protein phosphatase 4 promotes hepatic lipogenesis through dephosphorylating acetyl‑CoA carboxylase 1 on serine 79

Meng,Xiangyu; Li,Meng; Guo,Jun; Tang,Weiqing; Wang,Shu; Man,Yong; Huang,Xiuqing; Li,Jian

doi:10.3892/mmr.2014.2397

Protein phosphatase 4 promotes hepatic lipogenesis through dephosphorylating acetyl‑CoA carboxylase 1 on serine 79

Authors:
- Xiangyu Meng
- Meng Li
- Jun Guo
- Weiqing Tang
- Shu Wang
- Yong Man
- Xiuqing Huang
- Jian Li
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Affiliations: Peking University Fifth School of Clinical Medicine, Beijing Hospital, Ministry of Health, Beijing 100730, P.R. China, The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, P.R. China
Published online on: July 18, 2014 https://doi.org/10.3892/mmr.2014.2397
Pages: 1959-1963

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Abstract

Reversible phosphorylation has a critical role in the regulation of the activity of acetyl‑CoA carboxylase 1 (ACC1), which is associated with de novo lipogenesis. It has been shown that AMP‑activated protein kinase (AMPK) phosphorylates ACC1 on serine 79 and inhibits its activity; however, the mechanism of ACC1 dephosphorylation remains elusive. Protein phosphatase 4 (PP4), a ubiquitous serine/threonine phosphatase, regulates a variety of cellular functions; however, whether PP4 is involved in lipid metabolism has yet to be elucidated. In the present study, PP4 was identified as a novel regulator of ACC1, which is also involved in hepatic lipogenesis. The expression of PP4 was found to be significantly increased in the livers of db/db mice. Furthermore, pACC1‑Ser79/ACC1 levels were observed to be decreased and high triglyceride accumulation was found in the livers of db/db mice. Moreover, PP4 overexpression was observed to lead to a decreased pACC1‑Ser79/ACC1 ratio and subsequently an increased intracellular triglyceride content in mouse primary hepatocytes. PP4 was also found to directly interact with pACC1‑Ser79 in human HepG2 cells. In conclusion, the present study showed that PP4 may be a novel regulator in hepatic lipogenesis through dephosphorylating ACC1 on serine 79, suggesting that PP4 may be a promising therapeutic target in lipid metabolism disorders.

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