Open Access

Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells

  • Authors:
    • Yu Zhu
    • Nan Ma
    • Hui‑Xiang Li
    • Lin Tian
    • Yu‑Feng Ba
    • Bin Hao
  • View Affiliations

  • Published online on: July 21, 2014     https://doi.org/10.3892/mmr.2014.2405
  • Pages: 1734-1738
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Berberine, an isoquinoline alkaloid extracted from the dry root of Coptidis Rhizoma, has been found to exhibit marked anticancer effects on a panel of established cancer cells. Among the human osteosarcoma lines treated, MG‑63 cells were found to be the most sensitive. The present study investigated the potential genotoxic effect of berberine on MG‑63 human osteosarcoma cells. The effect of berberine on cell viability was determined using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay and cell apoptosis was analyzed by flow cytometry and a DNA ladder assay. γH2AX focus formation was used to detect DNA damage in MG‑63 cells. Berberine induced a significant increase in apoptosis in MG‑63 cells in a concentration‑ and time‑dependent manner, as determined by DNA fragmentation analysis and flow cytometry. Furthermore, berberine induced significant concentration‑ and time‑dependent increases in DNA damage compared with that in the negative control. In conclusion, these observations indicated that berberine induced apoptosis and DNA damage in MG‑63 cells.
View Figures
View References

Related Articles

Journal Cover

October 2014
Volume 10 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu Y, Ma N, Li HX, Tian L, Ba YF and Hao B: Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells. Mol Med Rep 10: 1734-1738, 2014.
APA
Zhu, Y., Ma, N., Li, H., Tian, L., Ba, Y., & Hao, B. (2014). Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells. Molecular Medicine Reports, 10, 1734-1738. https://doi.org/10.3892/mmr.2014.2405
MLA
Zhu, Y., Ma, N., Li, H., Tian, L., Ba, Y., Hao, B."Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells". Molecular Medicine Reports 10.4 (2014): 1734-1738.
Chicago
Zhu, Y., Ma, N., Li, H., Tian, L., Ba, Y., Hao, B."Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells". Molecular Medicine Reports 10, no. 4 (2014): 1734-1738. https://doi.org/10.3892/mmr.2014.2405