Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways

Liu,Bin; Zhou,Zhongyou; Zhou,Wei; Liu,Jie; Zhang,Qingyu; Xia,Juan; Liu,Juntao; Chen,Nianping; Li,Mingyi; Zhu,Runzhi

doi:10.3892/mmr.2014.2406

Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways

Authors:
- Bin Liu
- Zhongyou Zhou
- Wei Zhou
- Jie Liu
- Qingyu Zhang
- Juan Xia
- Juntao Liu
- Nianping Chen
- Mingyi Li
- Runzhi Zhu
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Affiliations: Laboratory of Hepatobiliary Surgery, Guangdong Medical College, Zhanjiang Key Laboratory of Hepatobiliary Diseases, Zhanjiang, Guangdong 524001, P.R. China, Department of Radiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China
Published online on: July 21, 2014 https://doi.org/10.3892/mmr.2014.2406
Pages: 1697-1702
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The present study compared the effect of resveratrol on HCT116 and Caco‑2 human colon cancer cells. Annexin V/propidium iodide staining, MTT assay and western blot analysis revealed that resveratrol induced cycle arrest in the two cell lines, which was evidenced by cell cycle analysis and changes in the expression of the cell cycle proteins cyclin‑dependent kinase (CDK) 2, CDK4, cyclin D1, proliferating cell nuclear antigen and P21. Furthermore, resveratrol was found to have a strong apoptosis‑inducing effect, which was evidenced through the high percentage of annexin V positive cells and high protein expression of cleaved‑caspase‑7, cleaved‑caspase‑9 and cleaved‑poly(ADP‑ribose) polymerase in the resveratrol‑treated cancer cells. In conclusion, these results demonstrated that resveratrol had greater growth inhibitory and cell cycle arrest effects on Caco‑2 cells than HCT116 cells, through caspase‑dependent and cyclin‑CDK pathways.

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