Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes

  • Authors:
    • Fei Xu
    • Yonghui Dong
    • Xin Huang
    • Mi Li
    • Liang Qin
    • Ye Ren
    • Fengjing Guo
    • Anmin Chen
    • Shilong Huang
  • View Affiliations

  • Published online on: July 30, 2014     https://doi.org/10.3892/mmr.2014.2430
  • Pages: 1935-1941
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Abstract

The effect of type 2 diabetes mellitus (T2DM) on bone is controversial. Therefore, the present study investigated whether T2DM causes osteoporosis and explored the underlying mechanisms involved in this process. The effects of T2DM on bone physiology were analyzed in a mouse model of T2DM; KK/Upj‑Ay/J (KK‑Ay) mice develop diabetes after 8 weeks and exhibit stable diabetes symptoms and signs after 10 weeks when fed a KK‑Ay mouse maintenance fodder. Diabetic mice exhibited hyperglycemia, hyperinsulinemia and increased body and fat pad weight in comparison with C57BL/6 non‑diabetic mice. Furthermore, diabetic mice demonstrated low bone weight and bone mineral density in the femur, tibia and fifth lumbar vertebra. Using von Kossa and tartrate‑resistant acid phosphatase (TRAP) staining, alkaline phosphatase and TRAP activity analyses and gene profiling it was demonstrated that osteoblastogenesis and osteoclastogenesis were impaired in diabetic mice. To evaluate the bone biomechanics, the ultimate load of the bone was analyzed. It was found that the ultimate load of the tibia in diabetic mice was lower than that in the controls. The results from the present study suggest that bone metabolism is impaired in T2DM, resulting in decreased osteoblastogenesis, osteoclastogenesis and bone mass.
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October 2014
Volume 10 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A and Huang S: Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes. Mol Med Rep 10: 1935-1941, 2014.
APA
Xu, F., Dong, Y., Huang, X., Li, M., Qin, L., Ren, Y. ... Huang, S. (2014). Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes. Molecular Medicine Reports, 10, 1935-1941. https://doi.org/10.3892/mmr.2014.2430
MLA
Xu, F., Dong, Y., Huang, X., Li, M., Qin, L., Ren, Y., Guo, F., Chen, A., Huang, S."Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes". Molecular Medicine Reports 10.4 (2014): 1935-1941.
Chicago
Xu, F., Dong, Y., Huang, X., Li, M., Qin, L., Ren, Y., Guo, F., Chen, A., Huang, S."Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes". Molecular Medicine Reports 10, no. 4 (2014): 1935-1941. https://doi.org/10.3892/mmr.2014.2430