A proteomics study of hyperhomocysteinemia injury of the hippocampal neurons using iTRAQ

  • Authors:
    • Min Fang
    • Jing Wang
    • Han Yan
    • Yan‑Xin Zhao
    • Xue‑Yuan Liu
  • View Affiliations

  • Published online on: September 11, 2014     https://doi.org/10.3892/mmr.2014.2557
  • Pages: 2511-2516
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Abstract

High levels of homocysteine, caused by abnormal methionine metabolism, can induce degeneration of mouse hippocampal neurons. iTRAQ™ technology has been widely used in the field of proteomics research and through employing this technology, the present study identified that hyperhomocysteinemia induced the downregulation of 52 proteins and upregulation of 44 proteins in the mouse hippocampus. Through gene ontology and pathway analysis, the upregulation of components of the cytoskeleton, actin, regulators of focal adhesion, calcium signaling pathways, tight junctions, ErbB and gonadotrophin‑releasing hormone signaling, leukocyte, transendothelial migration, propanoate and pyruvate metabolism, valine, leucine and isoleucine biosynthesis, synthesis and degradation of ketone bodies and benzoate degradation via CoA ligation pathway, was identified. It was additionally verified that tau protein was highly expressed in the hyperhomocysteinemic neurons. Further analysis revealed that tau network proteins played functional roles in homocysteine‑induced neuronal damage.
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November-2014
Volume 10 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Fang M, Wang J, Yan H, Zhao YX and Liu XY: A proteomics study of hyperhomocysteinemia injury of the hippocampal neurons using iTRAQ. Mol Med Rep 10: 2511-2516, 2014
APA
Fang, M., Wang, J., Yan, H., Zhao, Y., & Liu, X. (2014). A proteomics study of hyperhomocysteinemia injury of the hippocampal neurons using iTRAQ. Molecular Medicine Reports, 10, 2511-2516. https://doi.org/10.3892/mmr.2014.2557
MLA
Fang, M., Wang, J., Yan, H., Zhao, Y., Liu, X."A proteomics study of hyperhomocysteinemia injury of the hippocampal neurons using iTRAQ". Molecular Medicine Reports 10.5 (2014): 2511-2516.
Chicago
Fang, M., Wang, J., Yan, H., Zhao, Y., Liu, X."A proteomics study of hyperhomocysteinemia injury of the hippocampal neurons using iTRAQ". Molecular Medicine Reports 10, no. 5 (2014): 2511-2516. https://doi.org/10.3892/mmr.2014.2557