Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113

Shao,Yuan; Sha,Xiao‑Ying; Bai,Yan‑Xia; Quan,Fang; Wu,Sheng‑Li

doi:10.3892/mmr.2014.2717

Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113

Authors:
- Yuan Shao
- Xiao‑Ying Sha
- Yan‑Xia Bai
- Fang Quan
- Sheng‑Li Wu
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Affiliations: Department of Otorhinolaryngology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, The Sixth Hepatic Disease Ward, The Affiliated Xi'an Eighth Hospital, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: October 21, 2014 https://doi.org/10.3892/mmr.2014.2717
Pages: 212-218
Copyright: © Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The present study aimed to investigate the effect of A disintegrin and metalloproteinase 10 (ADAM10) gene silencing on the proliferation, migration and invasion of the human tongue squamous cell carcinoma cell line TCA8113. RNA interference was used to knock down the expression of ADAM10 in the TCA8113 cell line and the proliferation, migration and invasive ability of the treated cells were observed in vitro. The expression levels of epidermal growth factor receptor (EGFR) and E‑cadherin in the treated cells were determined by western blot analysis. The proliferation, migration and invasion abilities of cells in the ADAM10 siRNA‑treated group were significantly lower than those in the control groups (P<0.05). In addition, compared with the control groups, the expression levels of EGFR and E‑cadherin in the ADAM10 siRNA‑treated cells were significantly decreased (P<0.05) and increased (P<0.05), respectively. These results suggested that ADAM10 is important in regulating the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113 and that the mechanism may, at least in part, be associated with the upregulation of EGFR and the downregulation of E‑cadherin.

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1	Myers JN, Elkins T, Roberts D and Byers RM: Squamous cell carcinoma of the tongue in young adults: Increasing incidence and factors that predict treatment outcomes. Otolaryngol Head Neck Surg. 122:44–51. 2000. View Article : Google Scholar : PubMed/NCBI
2	Hernández-Guerrero JC, Jacinto-Alemán LF, Jiménez-Farfán MD, Macario-Hernández A, Hernández-Flores F and Alcántara-Vázquez A: Prevalence trends of oral squamous cell carcinoma. Mexico City’s General Hospital experience. Med Oral Patol Oral Cir Bucal. 18:e306–e311. 2013.PubMed/NCBI
3	Bagan J, Sarrion G and Jimenez Y: Oral cancer: clinical features. Oral Oncol. 46:414–417. 2010. View Article : Google Scholar
4	Haddad RI and Shin DM: Recent advances in head and neck cancer. N Engl J Med. 359:1143–1154. 2008. View Article : Google Scholar : PubMed/NCBI
5	Duffy MJ, Mullooly M, O’Donovan N, Sukor S, Crown J, Pierce A and McGowan PM: The ADAMs family of proteases: new biomarkers and therapeutic targets for cancer? Clin Proteomics. 8:92011. View Article : Google Scholar : PubMed/NCBI
6	Lin J, Luo J and Redies C: Differential expression of five members of the ADAM family in the developing chicken brain. Neuroscience. 157:360–375. 2008. View Article : Google Scholar : PubMed/NCBI
7	Murphy G: The ADAMs: signalling scissors in the tumour microenvironment. Nat Rev Cancer. 8:929–941. 2008. View Article : Google Scholar : PubMed/NCBI
8	Lu X, Lu D, Scully M and Kakkar V: ADAM proteins - therapeutic potential in cancer. Curr Cancer Drug Targets. 8:720–732. 2008. View Article : Google Scholar : PubMed/NCBI
9	Yuan S, Lei S and Wu S: ADAM10 is overexpressed in human hepatocellular carcinoma and contributes to the proliferation, invasion and migration of HepG2 cells. Oncol Rep. 30:1715–1722. 2013.PubMed/NCBI
10	Doberstein K, Pfeilschifter J and Gutwein P: The transcription factor PAX2 regulates ADAM10 expression in renal cell carcinoma. Carcinogenesis. 32:1713–1723. 2011. View Article : Google Scholar : PubMed/NCBI
11	Gaida MM, Haag N, Günther F, et al: Expression of A disintegrin and metalloprotease 10 in pancreatic carcinoma. Int J Mol Med. 26:281–288. 2010.PubMed/NCBI
12	Guo J, He L, Yuan P, et al: ADAM10 overexpression in human non-small cell lung cancer correlates with cell migration and invasion through the activation of the Notch1 signaling pathway. Oncol Rep. 28:1709–1718. 2012.PubMed/NCBI
13	Wang YY, Ye ZY, Li L, Zhao ZS, Shao QS and Tao HQ: ADAM 10 is associated with gastric cancer progression and prognosis of patients. J Surg Oncol. 103:116–123. 2011. View Article : Google Scholar : PubMed/NCBI
14	Prox J, Willenbrock M, Weber S, et al: Tetraspanin15 regulates cellular trafficking and activity of the ectodomain sheddase ADAM10. Cell Mol Life Sci. 69:2919–2932. 2012. View Article : Google Scholar : PubMed/NCBI
15	Turner SL, Blair-Zajdel ME and Bunning RA: ADAMs and ADAMTSs in cancer. Br J Biomed Sci. 66:117–128. 2009.PubMed/NCBI
16	Jones AV, Lambert DW, Speight PM and Whawell SA: ADAM 10 is over expressed in oral squamous cell carcinoma and contributes to invasive behaviour through a functional association with αvβ6 integrin. FEBS Lett. 587:3529–3534. 2013.PubMed/NCBI
17	Shao Y, Zhang SQ, Quan F, Zhang PF and Wu SL: MicroRNA-145 inhibits the proliferation, migration and invasion of the human TCA8113 oral cancer line. Oncol Lett. 6:1636–1640. 2013.PubMed/NCBI
18	Lee SB, Schramme A, Doberstein K, et al: ADAM10 is upregulated in melanoma metastasis compared with primary melanoma. J Invest Dermatol. 130:763–773. 2010. View Article : Google Scholar : PubMed/NCBI
19	Yu Y, Chen W, Zhang Y, Hamburger AW, Pan H and Zhang Z: Suppression of salivary adenoid cystic carcinoma growth and metastasis by ErbB3 binding protein Ebp1 gene transfer. Int J Cancer. 120:1909–1913. 2007. View Article : Google Scholar : PubMed/NCBI
20	Armanious H, Gelebart P, Anand M, Belch A and Lai R: Constitutive activation of metalloproteinase ADAM10 in mantle cell lymphoma promotes cell growth and activates the TNFα/NFκB pathway. Blood. 117:6237–6246. 2011.PubMed/NCBI
21	Zheng X, Jiang F, Katakowski M, Lu Y and Chopp M: ADAM17 promotes glioma cell malignant phenotype. Mol Carcinog. 51:150–164. 2012. View Article : Google Scholar : PubMed/NCBI
22	Ebsen H, Schröder A, Kabelitz D and Janssen O: Differential surface expression of ADAM10 and ADAM17 on human T lymphocytes and tumor cells. PLoS One. 8:e768532013. View Article : Google Scholar : PubMed/NCBI
23	Normanno N, De Luca A, Bianco C, et al: Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 366:2–16. 2006. View Article : Google Scholar : PubMed/NCBI
24	Yan Y, Shirakabe K and Werb Z: The metalloprotease Kuzbanian (ADAM10) mediates the transactivation of EGF receptor by G protein-coupled receptors. J Cell Biol. 158:221–226. 2002. View Article : Google Scholar : PubMed/NCBI
25	Millichip MI, Dallas DJ, Wu E, Dale S and McKie N: The metallo-disintegrin ADAM10 (MADM) from bovine kidney has type IV collagenase activity in vitro. Biochem Biophys Res Commun. 245:594–598. 1998. View Article : Google Scholar : PubMed/NCBI
26	Pan Y, Han C, Wang C, et al: ADAM10 promotes pituitary adenoma cell migration by regulating cleavage of CD44 and L1. J Mol Endocrinol. 49:21–33. 2012. View Article : Google Scholar : PubMed/NCBI
27	Solanas G, Cortina C, Sevillano M and Batlle E: Cleavage of E-cadherin by ADAM10 mediates epithelial cell sorting downstream of EphB signalling. Nat Cell Biol. 13:1100–1107. 2011. View Article : Google Scholar : PubMed/NCBI