Adiponectin reduces carotid atherosclerotic plaque formation in ApoE-/- mice: Roles of oxidative and nitrosative stress 
and inducible nitric oxide synthase

Cai,Xiaojun; Li,Xuan; Li,Li; Huang,Xiao-Zhen; Liu,Yu-Sheng; Chen,Liang; Zhang,Ke; Wang,Lin; Li,Xiaonan; Song,Jiantao; Li,Shuzhen; Zhang,Yun; Zhang,Mei

doi:10.3892/mmr.2014.2947

Adiponectin reduces carotid atherosclerotic plaque formation in ApoE-/- mice: Roles of oxidative and nitrosative stress and inducible nitric oxide synthase

Authors:
- Xiaojun Cai
- Xuan Li
- Li Li
- Xiao-Zhen Huang
- Yu-Sheng Liu
- Liang Chen
- Ke Zhang
- Lin Wang
- Xiaonan Li
- Jiantao Song
- Shuzhen Li
- Yun Zhang
- Mei Zhang
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Affiliations: The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Shandong University Qilu Hospital, Jinan, Shandong 250012, P.R. China, Department of Cardiology, Jinan Central Hospital Affiliated to Shandong University, Shandong 250014, P.R. China
Published online on: November 13, 2014 https://doi.org/10.3892/mmr.2014.2947
Pages: 1715-1721
Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Adiponectin (APN) is an important anti‑atherogenic adipocytokine. The aim of the present study was to investigate the role of adiponectin in atherosclerotic plaque formation and clarify its mechanisms. An atherosclerosis model was induced by in vivo perivascular constrictive silica collar placement on the left common carotid arteries in male apolipoprotein E‑deficient (ApoE‑/‑) mice. All of the mice were fed a high‑fat diet, and divided into phosphate‑buffered saline, adenovirus (Ad)‑β‑galactosidase and Ad‑APN treatment groups. Compared with treatment of Ad‑β‑gal or PBS, Ad‑APN treatment markedly reduced inducible nitric oxide synthase (iNOS) protein expression, decreased in nitric oxide/superoxide production, blocked peroxynitrite formation and reversed the progression of atherosclerotic lesions. Adiponectin may be a natural molecule that reduces atherosclerosis by inhibiting iNOS and consequently diminishing oxidative/nitrative stress.

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