Open Access

Effect of Melilotus extract on lung injury via the upregulation of tumor necrosis factor-α-induced protein-8-like 2 in septic mice

  • Authors:
    • Ming‑Wei Liu
    • Mei‑Xian Su
    • Yun‑Hui Wang
    • Chuan‑Yun Qian
  • View Affiliations

  • Published online on: November 17, 2014     https://doi.org/10.3892/mmr.2014.2965
  • Pages: 1675-1684
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

As a Traditional Chinese Medicine, Melilotus extracts have been reported to function as an anti‑inflammatory agent, antioxidant and inhibitor of capillary permeability. The present study aimed to identify the mechanisms by which Melilotus interferes with inflammation‑associated and oxidative stress pathways during sepsis. An animal model of cecal ligation‑perforation (CLP)‑induced sepsis was established. Two hours prior to surgery, animals in the treatment group were administered 25 mg/kg Melilotus extract tablets and subsequently every 8 h. At 24 h post‑administration, pathological modifications in lung tissue and expression levels of tumor necrosis factor‑α‑induced protein‑8‑like 2 (TIPE2) expression, nuclear factor (NF)‑κB, toll‑like receptor 4 (TLR4), heme oxygenase‑1 (HO‑1), inhibitor of κB kinase (IκB), pro‑inflammatory mediators (interleukin‑6 and tumor necrosis factor‑α), myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD), were examined. The results showed that Melilotus extract had a marked effect on the pathological manifestation of lung tissue and lung inflammatory response, the upregulation of TIPE2, HO‑1 and IκB expression, and the inhibition of TLR4 and NF‑κB activities. In addition, following treatment with Melilotus extract, the model animals demonstrated decreased levels of MPO and MDA as well as increased levels of SOD. In conclusion, these results indicated that Melilotus extract may be a potential therapeutic agent for the treatment of CLP‑induced lung injury, the mechanism of which proceeded via inflammation‑ and oxidation‑associated pathways by increasing TIPE2 expression.
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March-2015
Volume 11 Issue 3

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Spandidos Publications style
Liu MW, Su MX, Wang YH and Qian CY: Effect of Melilotus extract on lung injury via the upregulation of tumor necrosis factor-α-induced protein-8-like 2 in septic mice. Mol Med Rep 11: 1675-1684, 2015.
APA
Liu, M., Su, M., Wang, Y., & Qian, C. (2015). Effect of Melilotus extract on lung injury via the upregulation of tumor necrosis factor-α-induced protein-8-like 2 in septic mice. Molecular Medicine Reports, 11, 1675-1684. https://doi.org/10.3892/mmr.2014.2965
MLA
Liu, M., Su, M., Wang, Y., Qian, C."Effect of Melilotus extract on lung injury via the upregulation of tumor necrosis factor-α-induced protein-8-like 2 in septic mice". Molecular Medicine Reports 11.3 (2015): 1675-1684.
Chicago
Liu, M., Su, M., Wang, Y., Qian, C."Effect of Melilotus extract on lung injury via the upregulation of tumor necrosis factor-α-induced protein-8-like 2 in septic mice". Molecular Medicine Reports 11, no. 3 (2015): 1675-1684. https://doi.org/10.3892/mmr.2014.2965