Increased expression of endocan in arthritic synovial tissues: Effects of adiponectin on the expression of endocan in fibroblast-like synoviocytes

  • Authors:
    • Kyoung Soo Kim
    • Yeon-Ah Lee
    • Hye-In Ji
    • Ran Song
    • Jung Yeon Kim
    • Sang‑Hoon Lee
    • Seung-Jae Hong
    • Myung Chul Yoo
    • Hyung-In Yang
  • View Affiliations

  • Published online on: December 5, 2014     https://doi.org/10.3892/mmr.2014.3057
  • Pages: 2695-2702
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Abstract

This study was performed to evaluate whether endocan expression, which is known to be involved in tumor angiogenesis, was increased in rheumatoid arthritic tissues. In addition, the involvement of adiponectin in the regulation of endocan expression in arthritic joints was examined. Arthritic synovial tissues from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) were immunostained with antibodies to endocan and vascular endothelial growth factor (VEGF). Subsequently, synovial cells and human umbilical vein endothelial cells were cultured and stimulated with interleukin-1 β (IL-1β) or adiponectin. The mRNA and protein levels of endocan were evaluated by polymerase chain reaction and ELISA, respectively. Endocan expression was markedly increased in the inflammatory sites of RA synovial tissues. In OA tissues, endocan expression was higher in tissues displaying moderate and severe inflammation than in those with mild inflammation. In vitro expression levels of endocan and VEGF in endothelial and synovial cells were differentially increased in response to IL-1β stimulation. Adiponectin was a more potent stimulant of endocan than IL-1β at their respective physiological concentrations in synovial cells. Endocan silencing by small interfering RNA transfection of synovial cells decreased in vitro cell migration and invasion. In conclusion, adiponectin is an important factor in the stimulation of endocan expression in synovial cells. Adiponectin-induced endocan expression in synovial cells may stimulate cell migration and invasion as well as angiogenesis in the pannus of arthritic joints.
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April-2015
Volume 11 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kim KS, Lee Y, Ji H, Song R, Kim JY, Lee SH, Hong S, Yoo MC and Yang H: Increased expression of endocan in arthritic synovial tissues: Effects of adiponectin on the expression of endocan in fibroblast-like synoviocytes. Mol Med Rep 11: 2695-2702, 2015.
APA
Kim, K.S., Lee, Y., Ji, H., Song, R., Kim, J.Y., Lee, S. ... Yang, H. (2015). Increased expression of endocan in arthritic synovial tissues: Effects of adiponectin on the expression of endocan in fibroblast-like synoviocytes. Molecular Medicine Reports, 11, 2695-2702. https://doi.org/10.3892/mmr.2014.3057
MLA
Kim, K. S., Lee, Y., Ji, H., Song, R., Kim, J. Y., Lee, S., Hong, S., Yoo, M. C., Yang, H."Increased expression of endocan in arthritic synovial tissues: Effects of adiponectin on the expression of endocan in fibroblast-like synoviocytes". Molecular Medicine Reports 11.4 (2015): 2695-2702.
Chicago
Kim, K. S., Lee, Y., Ji, H., Song, R., Kim, J. Y., Lee, S., Hong, S., Yoo, M. C., Yang, H."Increased expression of endocan in arthritic synovial tissues: Effects of adiponectin on the expression of endocan in fibroblast-like synoviocytes". Molecular Medicine Reports 11, no. 4 (2015): 2695-2702. https://doi.org/10.3892/mmr.2014.3057