Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells

Wang,Long; Fan,Jing; Lin,Yan‑Shui; Guo,Yun‑Shan; Gao,Bo; Shi,Qi‑Yue; Wei,Bo‑Yuan; Chen,Li; Yang,Liu; Liu,Jian; Luo,Zhuo‑Jing

doi:10.3892/mmr.2014.3099

Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells

Authors:
- Long Wang
- Jing Fan
- Yan‑Shui Lin
- Yun‑Shan Guo
- Bo Gao
- Qi‑Yue Shi
- Bo‑Yuan Wei
- Li Chen
- Liu Yang
- Jian Liu
- Zhuo‑Jing Luo
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Affiliations: Department of Orthopedics, Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Orthopedics, First Affiliated Hospital, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, Laboratory of Molecular Endocrinology, Odense University Hospital, University of Southern Denmark, 5000 Odense C, Denmark
Published online on: December 16, 2014 https://doi.org/10.3892/mmr.2014.3099
Pages: 2711-2716

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Abstract

Glucocorticoid‑induced osteoporosis (GIOP) is a widespread clinical complication following glucocorticoid therapy. This irreversible damage to bone‑forming and ‑resorbing cells is essential in the pathogenesis of osteoporosis. Autophagy is a physiological process involved in the regulation of cells and their responses to diverse stimuli, however, the role of autophagy in glucocorticoid‑induced damage to bone marrow mesenchymal stem cells (BMSCs) remains unclear. The current study confirmed that glucocorticoid administration impaired the proliferation of BMSCs. Transmission electron microscopy, immunohistochemistry and western blot analysis detected autophagy in vitro and in GIOP model rats (in vivo). With the addition of the autophagy inhibitor 3‑methyladenine, the proliferative ability of BMSCs was further reduced, while the number of apoptotic BMSCs was significantly increased. The data suggests that in response to glucocorticoid administration, induced autophagy aids to maintain proliferation and prevent apoptosis of BMSCs. Thus, it is hypothesized that autophagy may be a novel target in the treatment or prevention of osteoporosis.

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