Open Access

Effect of small nuclear ribonucleoprotein-associated polypeptide N on the proliferation of medulloblastoma cells

  • Authors:
    • Junjie Jing
    • Yang Zhao
    • Chengfeng Wang
    • Qingshuang Zhao
    • Qinchuan Liang
    • Shousen Wang
    • Jie Ma
  • View Affiliations

  • Published online on: January 7, 2015     https://doi.org/10.3892/mmr.2015.3148
  • Pages: 3337-3343
  • Copyright: © Jing et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Spliceosome mutations have been reported in various types of cancer and a number of antitumor drugs have been observed to tightly bind to spliceosome components. Small nuclear ribonucleoprotein‑associated polypeptide N (SNRPN) is a small ribonuclear protein and is a key spliceosome constituent. However, the role of SNRPN in human medulloblastoma remains unknown. In the present study, the effect of SNRPN on cell growth was investigated in vitro using the Daoy human medulloblastoma cell line. Lentivirus (Lv)-mediated short hairpin (sh) RNA was used to silence SNRPN expression, which was verified by reverse transcription‑quantitative polymerase chain reaction and western blotting. Cell proliferation was examined by MTT and colony formation assays. Knockdown of SNRPN markedly reduced the proliferation and colony formation ability of Daoy medulloblastoma cells. In addition, flow cytometric analysis revealed that the cell cycle distribution was altered when the Daoy cells were infected with Lv‑shSNRPN. To the best of our knowledge, this is the first study to investigate the effect of SNRPN on cell proliferation in medulloblastoma. The results indicate that SNRPN may be a potential novel target for the development of pharmacological therapeutics in human medulloblastoma.
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May-2015
Volume 11 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Jing J, Zhao Y, Wang C, Zhao Q, Liang Q, Wang S and Ma J: Effect of small nuclear ribonucleoprotein-associated polypeptide N on the proliferation of medulloblastoma cells. Mol Med Rep 11: 3337-3343, 2015.
APA
Jing, J., Zhao, Y., Wang, C., Zhao, Q., Liang, Q., Wang, S., & Ma, J. (2015). Effect of small nuclear ribonucleoprotein-associated polypeptide N on the proliferation of medulloblastoma cells. Molecular Medicine Reports, 11, 3337-3343. https://doi.org/10.3892/mmr.2015.3148
MLA
Jing, J., Zhao, Y., Wang, C., Zhao, Q., Liang, Q., Wang, S., Ma, J."Effect of small nuclear ribonucleoprotein-associated polypeptide N on the proliferation of medulloblastoma cells". Molecular Medicine Reports 11.5 (2015): 3337-3343.
Chicago
Jing, J., Zhao, Y., Wang, C., Zhao, Q., Liang, Q., Wang, S., Ma, J."Effect of small nuclear ribonucleoprotein-associated polypeptide N on the proliferation of medulloblastoma cells". Molecular Medicine Reports 11, no. 5 (2015): 3337-3343. https://doi.org/10.3892/mmr.2015.3148