Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells

Nho,Kyoung Jin; Chun,Jin Mi; Kim,Dong‑Seon; Kim,Ho Kyoung

doi:10.3892/mmr.2015.3179

Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells

Authors:
- Kyoung Jin Nho
- Jin Mi Chun
- Dong‑Seon Kim
- Ho Kyoung Kim
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Affiliations: Herbal Medicine Resources Group, Korea Institute of Oriental Medicine, Yuseong‑gu, Daejeon 305‑811, Republic of Korea
Published online on: January 9, 2015 https://doi.org/10.3892/mmr.2015.3179
Pages: 3722-3728

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Abstract

Ampelopsis japonica (AJ) is a well‑known traditional oriental herb with anti‑inflammatory and anticancer activities. However, the molecular mechanisms by which AJ inhibits metastasis in breast cancer cells remain to be elucidated. The aim of the present study was to investigate the effects of AJ ethanol extract (EAJ) on highly metastatic human MDA‑MB‑231 breast cancer cells in vitro. AJ was extracted and chemically characterized. Cell proliferation was determined using a CCK‑8 assay and migration was detected using a wound healing motility assay. A Transwell assay was used to evaluate the invasion and metastatic capabilities of the MDA‑MB‑231 cells. In addition, the mRNA expression levels of metalloproteinase (MMP)‑2 and MMP‑9 and tissue inhibitors of metalloproteinases (TIMP)‑1 and TIMP‑2 were evaluated using reverse transcription quantitative polymerase chain reaction in vitro. The results of the present study characterized the signaling cascades that mediated the antimetastatic activity of AJ in the human MDA‑MB‑231 breast cancer cell line. EAJ significantly suppressed the migration and invasion of MDA‑MB‑231 cells in vitro and inhibited the expression of metalloproteinase (MMP)‑2 and MMP‑9. These findings identified the biological activity of EAJ in an in vitro model of cancer metastasis and provided a rationale for further investigation.

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1	Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar
2	Coughlin SS and Ekwueme DU: Breast cancer as a global health concern. Cancer Epidemiol. 33:315–318. 2009. View Article : Google Scholar : PubMed/NCBI
3	Weber GF: Molecular mechanisms of metastasis. Cancer Lett. 270:181–190. 2008. View Article : Google Scholar : PubMed/NCBI
4	Johansson N, Ahonen M and Kähäri VM: Matrix metalloproteinases in tumor invasion. Cell Mol Life Sci. 57:5–15. 2000. View Article : Google Scholar : PubMed/NCBI
5	Köhrmann A, Kammerer U, Kapp M, Dietl J and Anacker J: Expression of matrix metalloproteinases (MMPs) in primary human breast cancer and breast cancer cell lines. BMC Cancer. 9:1882009. View Article : Google Scholar
6	Wiwanitkit V: Thai ethnopharmacological herbs for diabetes treatment: data collection and informatics tracing for therapeutic property. Diabetes Metab Syndr. 5:103–104. 2011. View Article : Google Scholar : PubMed/NCBI
7	Kim JH, Kim MH, Yang G, Huh Y, Kim SH and Yang WM: Effects of topical application of Astragalus membranaceus on allergic dermatitis. Immunopharmacol Immunotoxicol. 35:151–156. 2013. View Article : Google Scholar
8	Han MH, Lee WS, Lu JN, et al: Citrus aurantium L. exhibits apoptotic effects on U937 human leukemia cells partly through inhibition of Akt. Int J Oncol. 40:2090–2096. 2012.PubMed/NCBI
9	Mahassni SH and Al-Reemi RM: Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (Lepidium sativum) seeds. Saudi J Biol Sci. 20:131–139. 2013. View Article : Google Scholar : PubMed/NCBI
10	Chang EJ, Lee WJ, Cho SH and Choi SW: Proliferative effects of flavan-3-ols and propelargonidins from rhizomes of Drynaria fortunei on MCF-7 and osteoblastic cells. Arch Pharm Res. 26:620–630. 2003. View Article : Google Scholar : PubMed/NCBI
11	Lu J, Kim SH, Jiang C, Lee H and Guo J: Oriental herbs as a source of novel anti-androgen and prostate cancer chemopreventive agents. Acta Pharmacol Sin. 28:1365–1372. 2007. View Article : Google Scholar : PubMed/NCBI
12	Hong CH, Hur SK, Oh OJ, Kim SS, Nam KA and Lee SK: Evaluation of natural products on inhibition of inducible cyclooxygenase (COX-2) and nitric oxide synthase (iNOS) in cultured mouse macrophage cells. J Ethnopharmacol. 83:153–159. 2002. View Article : Google Scholar : PubMed/NCBI
13	Bae KH: The medicinal Plants of Korea. Hwang JS: Korea: Kyo-Hak Publishing Co; 328. 2000
14	Suh BI and Jeong GY: Herbology. Lee JG: Korea: Dae Gu Hanny University; 108. 1987
15	Kim JH, Ju EM, Lee DK and Hwang HJ: Induction of apoptosis by momordin I in promyelocytic leukemia (HL-60) cells. Anticancer Res. 22:1885–1889. 2002.PubMed/NCBI
16	Koressaar T and Remm M: Enhancements and modifications of primer design program Primer3. Bioinformatics. 23:1289–1291. 2007. View Article : Google Scholar : PubMed/NCBI
17	Egeblad M and Werb Z: New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2:161–174. 2002. View Article : Google Scholar : PubMed/NCBI
18	Hong S, Park KK, Magae J, et al: Ascochlorin inhibits matrix metalloproteinase-9 expression by suppressing activator protein-1-mediated gene expression through the ERK1/2 signalling pathway: inhibitory effects of ascochlorin on the invasion of renal carcinoma cells. J Biol Chem. 280:25202–25209. 2005. View Article : Google Scholar : PubMed/NCBI
19	Cairns RA, Khokha R and Hill RP: Molecular mechanisms of tumor invasion and metastasis: an integrated view. Curr Mol Med. 3:659–671. 2003. View Article : Google Scholar : PubMed/NCBI
20	Sternlicht MD and Werb Z: How matrix metalloproteinases regulate cell behavior. Ann Rev Cell Dev Biol. 17:463–516. 2001. View Article : Google Scholar
21	Liotta LA and Stetler-Stevenson WG: Tumor invasion and metastasis: an imbalance of positive and negative regulation. Cancer Res. 51(18 Suppl): 5054s–5059s. 1991.PubMed/NCBI
22	Kato Y, Yamashita T and Ishikawa M: Relationship between expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and invasion ability of cervical cancer cells. Oncol Rep. 9:565–569. 2002.PubMed/NCBI
23	Gilabert-Estellés J, Ramón LA, España F, et al: Expression of angiogenic factors in endometriosis: relationship to fibrinolytic and metalloproteinase systems. Hum Reprod. 22:2120–2127. 2007. View Article : Google Scholar : PubMed/NCBI
24	Weng CJ, Yang YT, Ho CT and Yen GC: Mechanisms of apoptotic effects induced by resveratrol, dibenzoylmethane and their analogues on human lung carcinoma cells. J Agric Food Chem. 57:5235–5243. 2009. View Article : Google Scholar : PubMed/NCBI
25	Roy S, Sannigrahi S, Majumdar S, Ghosh B and Sarkar B: Resveratrol regulates antioxidant status, inhibits cytokine expression and restricts apoptosis in carbon tetrachloride induced rat hepatic injury. Oxid Med Cell Longev. 2011:7036762011. View Article : Google Scholar : PubMed/NCBI
26	Johnson WD, Morrissey RL, Usborne AL, et al: Subchronic oral toxicity and cardiovascular safety pharmacology studies of resveratrol, a naturally occurring polyphenol with cancer preventive activity. Food Chem Toxicol. 49:3319–3327. 2001. View Article : Google Scholar
27	Szekeres T, Saiko P, Fritzer-Szekeres M, Djavan B and Jäger W: Chemopreventive effects of resveratrol and resveratrol derivatives. Ann NY Acad Sci. 1215:89–95. 2011. View Article : Google Scholar : PubMed/NCBI
28	Fabre KM, Saito K, DeGraff W, et al: The effects of resveratrol and selected metabolites on the radiation and antioxidant response. Cancer Biol Ther. 12:915–923. 2011. View Article : Google Scholar : PubMed/NCBI
29	Scarlatti F, Sala G, Somenzi G, Signorelli P, Sacchi N and Ghidoni R: Resveratrol induces growth inhibition and apoptosis in metastatic breast cancer cells via de novo ceramide signaling. FASEB J. 17:2339–2341. 2003.PubMed/NCBI
30	Kundu JK and Surh YJ: Cancer chemopreventive and therapeutic potential of resveratrol, mechanistic perspectives. Cancer Lett. 269:243–261. 2008. View Article : Google Scholar : PubMed/NCBI
31	Castillo-Pichardo L and Dharmawardhane SF: Grape polyphenols inhibit Akt/mammalian target of rapamycin signaling and potentiate the effects of gefitinib in breast cancer. Nutr Cancer. 64:1058–1069. 2012. View Article : Google Scholar : PubMed/NCBI
32	Islam S, Nasrin S, Khan MA, et al: Evaluation of antioxidant and anticancer properties of the seed extracts of Syzygium fruticosum Roxb. growing in Rajshahi, Bangladesh. BMC Complement Altern Med. 13:1422013. View Article : Google Scholar : PubMed/NCBI
33	Yu Y, Deng Y, Lu BM, Liu YX, Li J and Bao JK: Green tea catechins: a fresh flavor to anticancer therapy. Apoptosis. 19:1–18. 2014. View Article : Google Scholar