Open Access

miR-34a inhibits migration and invasion by regulating the SIRT1/p53 pathway in human SW480 cells

  • Authors:
    • Mingguang Lai
    • Gang Du
    • Ruiyue Shi
    • Jun Yao
    • Genhua Yang
    • Yue Wei
    • Dingguo Zhang
    • Zhenglei Xu
    • Ru Zhang
    • Yingxue Li
    • Zicheng Li
    • Lisheng Wang
  • View Affiliations

  • Published online on: January 12, 2015     https://doi.org/10.3892/mmr.2015.3182
  • Pages: 3301-3307
  • Copyright: © Lai et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

MicroRNA-34a (miR-34a) is a direct transcriptional target of p53, and is downregulated in several different types of cancer. However, the underlying mechanism of the miR-34a effects in colorectal cancer is not well understood. In this study, we explored the role of miR-34a in cell invasion, migration, and apoptosis. Transient overexpression of miR-34a in SW480 cells caused a severe decrease in cell migration and invasion (both, p<0.05) compared to the control groups. Combining miR-34a transfection with 5-fluorouracil (5-FU) treatment further enhanced the inhibition in SW480 cell migration and invasion (both, p<0.05) compared to 5-FU treatment alone. These cellular changes were associated with upregulation of acetylated‑p53 (ac-p53) and p21 and downregulation of sirtuin 1 (SIRT1). These data demonstrate that miR-34a regulates the expression of a number of critical proteins involved in apoptosis, proliferation and the response to chemotherapy. In summary, miR-34a increases the sensitivity of colon cancer cells to 5-FU treatment through specific regulation of the SIRT1/p53 pathway.
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May-2015
Volume 11 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lai M, Du G, Shi R, Yao J, Yang G, Wei Y, Zhang D, Xu Z, Zhang R, Li Y, Li Y, et al: miR-34a inhibits migration and invasion by regulating the SIRT1/p53 pathway in human SW480 cells. Mol Med Rep 11: 3301-3307, 2015.
APA
Lai, M., Du, G., Shi, R., Yao, J., Yang, G., Wei, Y. ... Wang, L. (2015). miR-34a inhibits migration and invasion by regulating the SIRT1/p53 pathway in human SW480 cells. Molecular Medicine Reports, 11, 3301-3307. https://doi.org/10.3892/mmr.2015.3182
MLA
Lai, M., Du, G., Shi, R., Yao, J., Yang, G., Wei, Y., Zhang, D., Xu, Z., Zhang, R., Li, Y., Li, Z., Wang, L."miR-34a inhibits migration and invasion by regulating the SIRT1/p53 pathway in human SW480 cells". Molecular Medicine Reports 11.5 (2015): 3301-3307.
Chicago
Lai, M., Du, G., Shi, R., Yao, J., Yang, G., Wei, Y., Zhang, D., Xu, Z., Zhang, R., Li, Y., Li, Z., Wang, L."miR-34a inhibits migration and invasion by regulating the SIRT1/p53 pathway in human SW480 cells". Molecular Medicine Reports 11, no. 5 (2015): 3301-3307. https://doi.org/10.3892/mmr.2015.3182