Aldehyde dehydrogenase 1: A specific cancer stem cell marker for human colorectal carcinoma

Zhou,Fei; Mu,Yin‑Dong; Liang,Jun; Liu,Zhi‑Xin; Zhou,Dan; Ning,Wen‑Long; Li,Yue‑Zhen; Ding,Dong; Zhang,Ji‑Fei

doi:10.3892/mmr.2015.3195

Aldehyde dehydrogenase 1: A specific cancer stem cell marker for human colorectal carcinoma

Authors:
- Fei Zhou
- Yin‑Dong Mu
- Jun Liang
- Zhi‑Xin Liu
- Dan Zhou
- Wen‑Long Ning
- Yue‑Zhen Li
- Dong Ding
- Ji‑Fei Zhang
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Affiliations: Second Department of General Surgery, The First Hospital Qiqihar, Qiqihar, Heilongjiang 161000, P.R. China, Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang, Heilongjiang 157000, P.R. China, Department of Breast Cancer, The First Hospital of Foshan, Foshan, Guangdong 528000, P.R. China , Department of Emergency, The First Hospital, Qiqihar, Heilongjiang 161000, P.R. China, Life and Technology College Affiliated to Mudanjiang Teachers College, Mudanjiang, Heilongjiang, 157000, P.R. China
Published online on: January 13, 2015 https://doi.org/10.3892/mmr.2015.3195
Pages: 3894-3899

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Abstract

Since the identification of cancer stem cells (CSCs) a new understanding of tumor occurrence and development has evolved. According to the stem cell (SC) theory, colorectal carcinoma (CRC) SCs may be derived from mutations in normal intestinal cells. CSCs can be defined by their cell of origin (SCs or early progenitor cells). Thus, through a shared stem cell marker between CSCs and SCs, it is possible to investigate the association between its expression and the various clinicopathological features in patients with CRC. Aldehyde dehydrogenase 1 (ALDH1) is an appropriate marker. The present study was performed to examine the role of ALDH1 in CRC. Through indirect fluorescence antibody staining, the association between ALDH1 protein expression and various clinicopathological parameters was investigated. Furthermore, enzyme‑linked immunosorbent assay (ELISA) was used to investigate the differing content of ALDH1 between CRC tissues and normal colorectal tissues. The results revealed that ALDH1 expression was markedly associated with tumor stage, Dukes' stage and the level of tumor cell differentiation. Using ELISA, it was demonstrated that there was a greater level of ALDH1 in CRC tissue than in normal colorectal tissue. Therefore, ALDH1 levels can be used as a useful parameter for pathological evaluation of tissue histology and to predict disease prognosis.

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