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Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats

  • Authors:
    • Hongwei Li
    • Qi Wu
    • Long Xu
    • Xue Li
    • Jianmin Duan
    • Jingyan Zhan
    • Jing Feng
    • Xin Sun
    • Huaiyong Chen

  • View Affiliations

  • Published online on: January 21, 2015     https://doi.org/10.3892/mmr.2015.3234
  • Pages: 4639-4644
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Chronic obstructive pulmonary disease (COPD) is a major public health problem, and cigarette smoke (CS) is the primary risk factor. The pathology is often observed in the lung, but COPD is also associated with intestinal barrier disruption, although the underlying mechanisms are poorly understood. To address this, a CS‑exposed rat model was evaluated in the present study by analyzing small intestinal gene expression using reverse transcription‑quantitative polymerase chain reaction. CS exposure caused upregulation of the nicotinamide adenine dinucleotide phosphate‑oxidase subunits nox2 and p22phox in the small intestine, while the antioxidative enzyme superoxide dismutase was downregulated. CS exposure also increased bax expression and decreased bcl‑2 expression. This was associated with an elevation of hypoxia‑inducible factor (HIF)‑1α. Claudin‑1 was decreased and claudin‑2 increased, indicating a loosening of small intestinal tight junctions (TJs). These data suggest that during the development of COPD, HIF‑1α expression is altered in the small intestine, which may be associated with the increased oxidative stress and apoptosis, eventually resulting in disruption of the intestinal TJs.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Li H, Wu Q, Xu L, Li X, Duan J, Zhan J, Feng J, Sun X and Chen H: Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats. Mol Med Rep 11: 4639-4644, 2015.
APA
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J. ... Chen, H. (2015). Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats. Molecular Medicine Reports, 11, 4639-4644. https://doi.org/10.3892/mmr.2015.3234
MLA
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J., Feng, J., Sun, X., Chen, H."Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats". Molecular Medicine Reports 11.6 (2015): 4639-4644.
Chicago
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J., Feng, J., Sun, X., Chen, H."Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats". Molecular Medicine Reports 11, no. 6 (2015): 4639-4644. https://doi.org/10.3892/mmr.2015.3234