Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway

Niu,Yin‑Bo; Kong,Xiang‑He; Li,Yu‑Hua; Fan,Li; Pan,Ya‑Lei; Li,Chen‑Rui; Wu,Xiang‑Long; Lu,Ting‑Li; Mei,Qi‑Bing

doi:10.3892/mmr.2015.3249

Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway

Authors:
- Yin‑Bo Niu
- Xiang‑He Kong
- Yu‑Hua Li
- Li Fan
- Ya‑Lei Pan
- Chen‑Rui Li
- Xiang‑Long Wu
- Ting‑Li Lu
- Qi‑Bing Mei
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Affiliations: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, P.R. China, Graduate School of Chang'an University, Xi'an, Shaanxi 710064, P.R. China, Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
Published online on: January 26, 2015 https://doi.org/10.3892/mmr.2015.3249
Pages: 4468-4472

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Abstract

Radix Dipsaci total saponins (RTS) are primary active components of Radix Dipsaci, which is administered orally for the treatment of osteoporosis according to Chinese Medicine. RTS have also been shown to reduce the risk of bone fractures in rats. However, the detailed molecular mechanisms underlying their action remain elusive. In the present study, the ability of RTS to increase alkaline phosphatase activity, osteocalcin levels and the degree of mineralization was investigated in MC3T3‑E1 mouse osteoblast precursor cells. In addition, the associated molecular mechanism was detected. The results revealed that RTS exerted an effect on osteoblastic maturation and differentiation. Induction of differentiation by RTS was associated with an increase in the expression levels of bone morphogenetic protein‑2 (BMP‑2), phosphorylated (P)‑Smad1/5/8, P‑ERK1/2, P‑p38 and Runt‑related transcription factor 2 (Runx2). Blocking BMP‑2 expression with noggin significantly reduced the levels of osteoblastic differentiation and subsequently attenuated the expression levels of P‑Smad1/5/8, P‑ERK1/2, P‑p38 and Runx2. This indicated that RTS induced osteoblastic differentiation through BMP‑2/mitogen‑activated protein kinase/Smad1/5/8‑dependent Runx2 signaling pathways and that it may be a promising agent for enhancing bone formation.

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