Association of hsa-miR‑145 overexpression in human testicular cells with male infertility

Zhang,Liyuan; Ding,Xianping; Nie,Shuangshuang; Li‑Ling,Jesse; Zhang,Yuping; Zhang,Hui; Chen,Lin; Li,Lingxiao; Ding,Min

doi:10.3892/mmr.2015.3273

Association of hsa-miR‑145 overexpression in human testicular cells with male infertility

Authors:
- Liyuan Zhang
- Xianping Ding
- Shuangshuang Nie
- Jesse Li‑Ling
- Yuping Zhang
- Hui Zhang
- Lin Chen
- Lingxiao Li
- Min Ding
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Affiliations: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China
Published online on: January 29, 2015 https://doi.org/10.3892/mmr.2015.3273
Pages: 4365-4372

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Abstract

MicroRNAs (miRs) have crucial functions in spermatogenesis and implications for male infertility. In the present study, Homo sapiens (hsa)‑miR‑145 was designed and cloned into the eukaryotic expression plasmid pGenesil‑1. The recombinant plasmids were transfected into Hs 1.tes normal testicular cells and NTERA‑2 testicular cancer cells. Quantitative polymerase chain reaction of hsa‑miR‑145 indicated that pGenesil‑1‑miR‑145 effectively upregulated the expression of hsa‑miR‑145 in vitro. hsa‑miR‑145 overexpression inhibited the mRNA and protein expression of sex-determining region Y Box 9 in Hs 1.tes cells. The proliferation rates of NTERA‑2 cells transfected with pGenesil‑1‑miR‑145 were significantly decreased. High expression levels of miR‑145 promoted cell apoptosis in NTERA‑2 cells. The results revealed that altered hsa‑miR‑145 expression in testicular cells affects the regulation of target genes associated with male infertility.

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