Matrine inhibits the migratory and invasive properties of nasopharyngeal carcinoma cells

Sun,Bin; Xu,Min

doi:10.3892/mmr.2015.3276

Matrine inhibits the migratory and invasive properties of nasopharyngeal carcinoma cells

Authors:
- Bin Sun
- Min Xu
View Affiliations

Affiliations: Department of Otolaryngology-Head and Neck Surgery, Second Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: January 29, 2015 https://doi.org/10.3892/mmr.2015.3276
Pages: 4158-4164
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Matrine is a widely used Chinese herbal medicine that has historically been used in the treatment of inflammation and cancer. However, the antimetastatic effects and associated molecular mechanisms of matrine on nasopharyngeal carcinoma (NPC) remain to be elucidated. Therefore, the aims of the present study were to assess the antimetastatic effects of matrine on NPC, and identify the underlying mechanisms. Matrine inhibited the proliferation of NPC cells in vitro and in vivo. Furthermore, matrine inhibited the migration and invasion of NPC tumor cells at doses below the toxic range. Following treatment with matrine for 24 h, there was a decrease in the protein expression levels and activities of matrix metalloproteinase (MMP)‑2 and MMP‑9 in NPC‑039 cells. In addition, matrine markedly reduced the expression levels of p65 and p50 in the nuclei. Combined treatment of matrine with helenalin, a nuclear factor‑κB (NF‑κB) inhibitor resulted in a synergistic reduction in MMP‑2 and MMP‑9 expression levels, and the invasive capabilities of the NPC‑039 cells were also reduced. In conclusion, matrine inhibits NPC cell migration and invasion by suppressing the NF‑κB pathway. These results suggest that matrine may be a potential therapeutic agent for NPC.

View Figures

View References

1	Albeck H, Bentzen J, Ockelmann HH, Nielsen NH, Bretlau P and Hansen HS: Familial clusters of nasopharyngeal carcinoma and salivary gland carcinomas in Greenland natives. Cancer. 72:196–200. 1993. View Article : Google Scholar : PubMed/NCBI
2	Liu A, Huang W, Zeng G, Ma X, Zhou X, Wang Y, Ouyang C and Cheng A: Expression of the Annexin A1 gene is associated with suppression of growth, invasion and metastasis of nasopharyngeal carcinoma. Mol Med Rep. 10:3059–67. 2014.PubMed/NCBI
3	Wei WI and Sham JS: Nasopharyngeal carcinoma. Lancet. 365:2041–2054. 2005. View Article : Google Scholar : PubMed/NCBI
4	Chua DT, Sham JS, Wei WI, Ho WK and Au GK: The predictive value of the 1997 american joint committee on cancer stage classification in determining failure patterns in nasopharyngeal carcinoma. Cancer. 92:2845–2855. 2001. View Article : Google Scholar : PubMed/NCBI
5	Cheng SH, Jian JJ, Tsai SY, et al: Prognostic features and treatment outcome in locoregionally advanced nasopharyngeal carcinoma following concurrent chemotherapy and radiotherapy. Int J Radiat Oncol Biol Phys. 41:755–762. 1998. View Article : Google Scholar : PubMed/NCBI
6	Chen K, Zhang S, Ji Y, et al: Baicalein inhibits the invasion and metastatic capabilities of hepatocellular carcinoma cells via down-regulation of the ERK pathway. PLoS One. 8:e729272013. View Article : Google Scholar : PubMed/NCBI
7	Raghu H, Sodadasu PK, Malla RR, Gondi CS, Estes N and Rao JS: Localization of uPAR and MMP-9 in lipid rafts is critical for migration, invasion and angiogenesis in human breast cancer cells. BMC Cancer. 10:6472010. View Article : Google Scholar : PubMed/NCBI
8	Bjorklund M and Koivunen E: Gelatinase-mediated migration and invasion of cancer cells. Biochim Biophys Acta. 1755:37–69. 2005.PubMed/NCBI
9	Yang SF, Hsieh YS, Lin CL, et al: Increased plasma levels of urokinase plasminogen activator and matrix metalloproteinase-9 in nonsmall cell lung cancer patients. Clin Chim Acta. 354:91–99. 2005. View Article : Google Scholar : PubMed/NCBI
10	Li Y, Wang B, Zhou C and Bi Y: Matrine induces apoptosis in angiotensin II-stimulated hyperplasia of cardiac fibroblasts: effects on Bcl-2/Bax expression and caspase-3 activation. Basic Clin Pharmacol Toxicol. 101:1–8. 2007. View Article : Google Scholar : PubMed/NCBI
11	Long Y, Lin XT, Zeng KL and Zhang L: Efficacy of intramuscular matrine in the treatment of chronic hepatitis B. Hepatobiliary Pancreat Dis Int. 3:69–72. 2004.PubMed/NCBI
12	Haiyan W, Yuxiang L, Linglu D, et al: Antinociceptive effects of matrine on neuropathic pain induced by chronic constriction injury. Pharm Biol. 51:844–850. 2013. View Article : Google Scholar : PubMed/NCBI
13	Li X, Zhou R, Zheng P, et al: Cardioprotective effect of matrine on isoproterenol-induced cardiotoxicity in rats. J Pharm Pharmacol. 62:514–520. 2010.PubMed/NCBI
14	Li Y, Zhang J, Ma H, et al: Protective role of autophagy in matrine-induced gastric cancer cell death. Int J Oncol. 42:1417–1426. 2013.PubMed/NCBI
15	Guo L, Xue TY, Xu W and Gao JZ: Effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells. Zhongguo Dang Dai Er Ke Za Zhi. 14:780–784. 2012.In Chinese. PubMed/NCBI
16	Zhang S, Zhang Y, Zhuang Y, et al: Matrine induces apoptosis in human acute myeloid leukemia cells via the mitochondrial pathway and Akt inactivation. PLoS One. 7:e468532012. View Article : Google Scholar : PubMed/NCBI
17	Li LQ, Li XL, Wang L, et al: Matrine inhibits breast cancer growth via miR-21/PTEN/Akt pathway in MCF-7 cells. Cell Physiol Biochem. 30:631–641. 2012. View Article : Google Scholar : PubMed/NCBI
18	Li M, Su BS, Chang LH, et al: Oxymatrine induces apoptosis in human cervical cancer cells through guanine nucleotide depletion. Anticancer Drugs. 25:161–173. 2014. View Article : Google Scholar
19	Yu HB, Zhang HF, Li DY, Zhang X, Xue HZ and Zhao SH: Matrine inhibits matrix metalloproteinase-9 expression and invasion of human hepatocellular carcinoma cells. J Asian Nat Prod Res. 13:242–250. 2011. View Article : Google Scholar : PubMed/NCBI
20	Yu P, Liu Q, Liu K, Yagasaki K, Wu E and Zhang G: Matrine suppresses breast cancer cell proliferation and invasion via VEGF-Akt-NF-kappaB signaling. Cytotechnology. 59:219–229. 2009. View Article : Google Scholar : PubMed/NCBI
21	Zhang W, Dai BT and Xu YH: Effects of matrine on invasion and metastasis of leukemia cell line jurkat. Zhongguo Zhong Xi Yi Jie He Za Zhi. 28:907–911. 2008.In Chinese. View Article : Google Scholar
22	Song S, Zhu S, Zhang Z, Mo Z, Ke Q and Luo Z: A study on the inhibitory effect of matrine on gastric cancer SGC-7901 cells. Afr J Tradit Complement Altern Med. 10:435–438. 2013. View Article : Google Scholar : PubMed/NCBI
23	Chang C, Liu SP, Fang CH, et al: Effects of matrine on the proliferation of HT29 human colon cancer cells and its antitumor mechanism. Oncol Lett. 6:699–704. 2013.PubMed/NCBI
24	Gialeli C, Theocharis AD and Karamanos NK: Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting. FEBS J. 278:16–27. 2011. View Article : Google Scholar
25	Yeh CB, Hsieh MJ, Hsieh YH, Chien MH, Chiou HL and Yang SF: Antimetastatic effects of norcantharidin on hepatocellular carcinoma by transcriptional inhibition of MMP-9 through modulation of NF-kB activity. PLoS One. 7:e310552012. View Article : Google Scholar : PubMed/NCBI
26	Yeh CB, Hsieh MJ, Hsieh YS, et al: Terminalia catappa exerts antimetastatic effects on hepatocellular carcinoma through transcriptional inhibition of matrix metalloproteinase-9 by modulating NF-kappaB and AP-1 activity. Evid Based Complement Alternat Med. 2012:5952922012. View Article : Google Scholar
27	Zhang YJ, Xiang MX, San J, Cheng G and Wang SS: Effect of matrine and carvedilol on collagen and MMPs activity of hypertrophy myocardium induced by pressure overload. J Zhejiang Univ Sci B. 7:245–250. 2006. View Article : Google Scholar : PubMed/NCBI
28	Gao H, He S, Tang WX and Wang J: Inhibition of NF-kappa B activity enhanced apoptosis induced by matrine in hepatocellular carcinoma cells. Zhonghua Gan Zang Bing Za Zhi. 15:914–917. 2007.In Chinese.
29	Hu H, Wang S, Zhang C, et al: Synthesis and in vitro inhibitory activity of matrine derivatives towards pro-inflammatory cytokines. Bioorg Med Chem Lett. 20:7537–7539. 2010. View Article : Google Scholar : PubMed/NCBI
30	Zhang B, Liu ZY, Li YY, et al: Antiinflammatory effects of matrine in LPS-induced acute lung injury in mice. Eur J Pharm Sci. 44:573–579. 2011. View Article : Google Scholar : PubMed/NCBI
31	Xu M, Yang L, Hong LZ, Zhao XY and Zhang HL: Direct protection of neurons and astrocytes by matrine via inhibition of the NF-kappaB signaling pathway contributes to neuroprotection against focal cerebral ischemia. Brain Res. 1454:48–64. 2012. View Article : Google Scholar : PubMed/NCBI