Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway

  • Authors:
    • Qun Lu
    • Wenjie Mei
    • Shaohong Luo
    • Weibin He
  • View Affiliations

  • Published online on: February 4, 2015     https://doi.org/10.3892/mmr.2015.3297
  • Pages: 4424-4430
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Abstract

A ruthenium(II) complex coordinated by cordycepin, [Cor‑Ru(II)], was synthesized for investigation as a potential novel candidate for cancer therapy. The antitumor activity of Cor‑Ru(II) was investigated by MTT and flow cytometry (FCM) assays. The results showed that Cor‑Ru(II) significantly inhibited the proliferation of Bel‑7402 human hepatoma cells and delayed cell cycle progression. Subsequent experiments using FCM and western blot analysis indicated that Cor‑Ru(II) induced cell apoptosis via suppression of bcl‑2 expression and stimulation of p53 expression. Cor‑Ru(II) was shown to interfere with the synthesis of DNA. This was confirmed by the in vitro binding to DNA in the groove binding mode (Kb=4.22(±0.2)x105 M‑1). Thus, Cor‑Ru(II) appears to act as an effective antitumor agent in vitro.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu Q, Mei W, Luo S and He W: Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway. Mol Med Rep 11: 4424-4430, 2015.
APA
Lu, Q., Mei, W., Luo, S., & He, W. (2015). Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway. Molecular Medicine Reports, 11, 4424-4430. https://doi.org/10.3892/mmr.2015.3297
MLA
Lu, Q., Mei, W., Luo, S., He, W."Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway". Molecular Medicine Reports 11.6 (2015): 4424-4430.
Chicago
Lu, Q., Mei, W., Luo, S., He, W."Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway". Molecular Medicine Reports 11, no. 6 (2015): 4424-4430. https://doi.org/10.3892/mmr.2015.3297