Open Access

Zinc ion implantation‑deposition technique improves the osteoblast biocompatibility of titanium surfaces

  • Authors:
    • Yongqiang Liang
    • Juan Xu
    • Jing Chen
    • Mengchun Qi
    • Xuehong Xie
    • Min Hu
  • View Affiliations

  • Published online on: February 6, 2015     https://doi.org/10.3892/mmr.2015.3311
  • Pages: 4225-4231
  • Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The plasma immersion ion implantation and deposition (PIIID) technique was used to implant zinc (Zn) ions into smooth surfaces of pure titanium (Ti) disks for investigation of tooth implant surface modification. The aim of the present study was to evaluate the surface structure and chemical composition of a modified Ti surface following Zn ion implantation and deposition and to examine the effect of such modification on osteoblast biocompatibility. Using the PIIID technique, Zn ions were deposited onto the smooth surface of pure Ti disks. The physical structure and chemical composition of the modified surface layers were characterized by scanning electron microscopy (SEM) and X‑ray photoelectron spectroscopy (XPS), respectively. In vitro culture assays using the MG‑63 bone cell line were performed to determine the effects of Zn‑modified Ti surfaces following PIIID on cellular function. Acridine orange staining was used to detect cell attachment to the surfaces and cell cycle analysis was performed using flow cytometry. SEM revealed a rough ‘honeycomb’ structure on the Zn‑modified Ti surfaces following PIIID processing and XPS data indicated that Zn and oxygen concentrations in the modified Ti surfaces increased with PIIID processing time. SEM also revealed significantly greater MG‑63 cell growth on Zn‑modified Ti surfaces than on pure Ti surfaces (P<0.05). Flow cytometric analysis revealed increasing percentages of MG‑63 cells in S phase with increasing Zn implantation and deposition, suggesting that MG‑63 apoptosis was inhibited and MG‑63 proliferation was promoted on Zn‑PIIID‑Ti surfaces. The present results suggest that modification with Zn‑PIIID may be used to improve the osteoblast biocompatibility of Ti implant surfaces.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liang Y, Xu J, Chen J, Qi M, Xie X and Hu M: Zinc ion implantation‑deposition technique improves the osteoblast biocompatibility of titanium surfaces. Mol Med Rep 11: 4225-4231, 2015.
APA
Liang, Y., Xu, J., Chen, J., Qi, M., Xie, X., & Hu, M. (2015). Zinc ion implantation‑deposition technique improves the osteoblast biocompatibility of titanium surfaces. Molecular Medicine Reports, 11, 4225-4231. https://doi.org/10.3892/mmr.2015.3311
MLA
Liang, Y., Xu, J., Chen, J., Qi, M., Xie, X., Hu, M."Zinc ion implantation‑deposition technique improves the osteoblast biocompatibility of titanium surfaces". Molecular Medicine Reports 11.6 (2015): 4225-4231.
Chicago
Liang, Y., Xu, J., Chen, J., Qi, M., Xie, X., Hu, M."Zinc ion implantation‑deposition technique improves the osteoblast biocompatibility of titanium surfaces". Molecular Medicine Reports 11, no. 6 (2015): 4225-4231. https://doi.org/10.3892/mmr.2015.3311