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Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats

  • Authors:
    • Wang Yang
    • Meng Xv
    • Wan Chao Yang
    • Nan Wang
    • Xue Zhong Zhang
    • Wen Zhi Li

  • View Affiliations

  • Published online on: April 15, 2015     https://doi.org/10.3892/mmr.2015.3620
  • Pages: 2181-2188
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Abstract

α-Calcitonin gene-related peptide (α-CGRP) is a 37 amino-acid neuropeptide that is primarily released from C‑type sensory neurons. α‑CGRP exerts multiple modulatory effects on immune responses and visceral organ function, but the role of exogenous α‑CGRP in lipopolysaccharide (LPS)‑induced acute lung injury (ALI) has remained to be elucidated. Forty‑eight rats were randomized to receive continuous intraperitoneal infusion of α‑CGRP (0.4 µg/kg/min) or normal saline for 30 min, followed by intratracheal injection of 0.5 mg/kg LPS or saline. There were four groups of animals: The saline‑saline (S‑S) group; the saline‑α‑CGRP (S‑C) group; the LPS‑saline (L‑S) group and the LPS‑α‑CGRP (L‑C) group. Mean arterial pressure and arterial blood gases were assessed prior to α‑CGRP and LPS administration and every hour following LPS treatment. After 4 h, bronchoalveolar lavage was performed and used to assess total cell count and levels of tumor necrosis factor‑α, interleukin‑1β, intracellular cell adhesion molecule 1 and macrophage inflammatory protein 2. Lung tissue was also collected for assessing wet‑to‑dry (W/D) ratio, histology and Evans blue (EB) dye extravasation. Pulmonary α‑CGRP concentration and α‑CGRP receptor expression were also examined, and inducible cyclic adenosine monophosphate early repressor (ICER) and TNF‑α mRNA expression levels were measured. Treatment with exogenous α‑CGRP improved oxygenation during LPS‑induced ALI. Correspondingly, histological injury, total cell count, inflammatory cytokine levels, W/D ratio and EB dye extravasation were also significantly reduced. α‑CGRP receptor 1 expression was noted in pulmonary endothelial cells and alveolar macrophages and α‑CGRP receptor expression levels were decreased during ALI, whereas pulmonary α‑CGRP expression was continuously increased. Furthermore, exogenous α‑CGRP induced upregulation of ICER during LPS‑induced ALI. In conclusion, exogenous α‑CGRP improved oxygenation and ameliorated lung damage in LPS‑induced ALI, and these effects were associated with the upregulation of ICER.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Yang W, Xv M, Yang WC, Wang N, Zhang XZ and Li WZ: Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats. Mol Med Rep 12: 2181-2188, 2015.
APA
Yang, W., Xv, M., Yang, W.C., Wang, N., Zhang, X.Z., & Li, W.Z. (2015). Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats. Molecular Medicine Reports, 12, 2181-2188. https://doi.org/10.3892/mmr.2015.3620
MLA
Yang, W., Xv, M., Yang, W. C., Wang, N., Zhang, X. Z., Li, W. Z."Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats". Molecular Medicine Reports 12.2 (2015): 2181-2188.
Chicago
Yang, W., Xv, M., Yang, W. C., Wang, N., Zhang, X. Z., Li, W. Z."Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats". Molecular Medicine Reports 12, no. 2 (2015): 2181-2188. https://doi.org/10.3892/mmr.2015.3620