Downregulation of microRNA‑221 decreases migration and invasion in fibroblast‑like synoviocytes in rheumatoid arthritis

Yang,Shuzhong; Yang,Yougeng

doi:10.3892/mmr.2015.3642

Downregulation of microRNA‑221 decreases migration and invasion in fibroblast‑like synoviocytes in rheumatoid arthritis

Authors:
- Shuzhong Yang
- Yougeng Yang
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Affiliations: Department of Orthopaedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China
Published online on: April 17, 2015 https://doi.org/10.3892/mmr.2015.3642
Pages: 2395-2401

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Abstract

MicroRNAs (miRNAs/miRs) are a group of non‑coding RNAs that regulate the activity of target mRNAs and cellular processes, and which have been implicated in the pathogenesis of autoimmune diseases. miR‑221 is one of the miRNAs that regulate cell proliferation, invasion and apoptosis in tumors. However, the role of miR‑221 in rheumatoid arthritis (RA) remains to be fully elucidated. Therefore, the present study was undertaken to identify the role of miR‑221 in RA. The expression of miR‑221 in serum and synovial tissues of patients with RA and healthy controls was confirmed by reverse transcription quantitative polymerase chain reaction analysis. The effects of miR‑221 on pro‑inflammatory cytokines and a chemokine were assessed by ELISA. The effects of miR‑221 on cell apoptosis, migration and invasion in fibroblast‑like synoviocytes (FLS) were also assessed in vitro. The results showed that miR‑221 expression in serum and synovial tissues of patients with RA was higher than that in healthy controls. Downregulation of miR‑221 significantly suppressed the expression of pro‑inflammatory cytokines and the chemokine, and inhibited FLS cell migration and invasion via inhibiting vascular endothelial growth factor, matrix metalloproteinase (MMP)‑3 and MMP‑9 expression. In addition, downregulation of miR‑221 significantly induced cell apoptosis and decreased survivin and X‑linked inhibitor of apoptosis protein expression. These findings indicated that downregulation of miR‑221 inhibited the expression of pro‑inflammatory cytokines and the chemokine, suppressed FLS cell migration and invasion, and induced cell apoptosis. Therefore, miR‑221 is likely to be implicated in RA pathogenesis via these mechanisms, and may be a target for the treatment of RA.

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