Secreted protein acidic and rich in cysteine antagonizes bufalin‑induced apoptosis in gastric cancer cells

  • Authors:
    • Aodi Li
    • Xiujuan Qu
    • Zhi Li
    • Jinglei Qu
    • Na Song
    • Yanju Ma
    • Yunpeng Liu
  • View Affiliations

  • Published online on: April 24, 2015     https://doi.org/10.3892/mmr.2015.3676
  • Pages: 2926-2932
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Abstract

Bufalin is an active compound in the traditional Chinese medicine Chan Su, which has been shown to induce apoptosis in a range of cancer cell types. However, certain gastric cancer cells are known to be resistant to bufalin. Intracellular secreted protein acidic and rich in cysteine (SPARC) regulates proliferation and apoptosis. This study aimed to evaluate the role of SPARC in bufalin‑induced apoptosis in SGC7901 and MGC803 gastric cancer cells. SGC7901 cells with high SPARC expression were more resistant to bufalin than MGC803 cells with low SPARC expression. This resistance was significantly reversed by small interfering (si)RNA‑mediated knockdown of SPARC. Furthermore, it was shown that SPARC negatively regulated bufalin‑induced intrinsic apoptosis by protecting mitochondrial integrity, decreasing the release of cytoplasmic cytochrome c and increasing the ratio of Bcl‑2/Bax. In addition, SPARC overcame bufalin‑induced G2/M phase arrest by increasing levels of Cyclin B1 and Cyclin A protein expression. SPARC also activated cellular survival signals, including Src and Akt, but not extracellular signal‑regulated kinase. This study demonstrated that SPARC antagonizes bufalin‑induced apoptosis via inhibition of the intrinsic apoptosis pathway, inhibition of cell cycle arrest and activation of certain pathways involved in proliferation. This provides novel evidence for SPARC as a potential target by which to sensitize gastric cancer cells to bufalin.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li A, Qu X, Li Z, Qu J, Song N, Ma Y and Liu Y: Secreted protein acidic and rich in cysteine antagonizes bufalin‑induced apoptosis in gastric cancer cells. Mol Med Rep 12: 2926-2932, 2015.
APA
Li, A., Qu, X., Li, Z., Qu, J., Song, N., Ma, Y., & Liu, Y. (2015). Secreted protein acidic and rich in cysteine antagonizes bufalin‑induced apoptosis in gastric cancer cells. Molecular Medicine Reports, 12, 2926-2932. https://doi.org/10.3892/mmr.2015.3676
MLA
Li, A., Qu, X., Li, Z., Qu, J., Song, N., Ma, Y., Liu, Y."Secreted protein acidic and rich in cysteine antagonizes bufalin‑induced apoptosis in gastric cancer cells". Molecular Medicine Reports 12.2 (2015): 2926-2932.
Chicago
Li, A., Qu, X., Li, Z., Qu, J., Song, N., Ma, Y., Liu, Y."Secreted protein acidic and rich in cysteine antagonizes bufalin‑induced apoptosis in gastric cancer cells". Molecular Medicine Reports 12, no. 2 (2015): 2926-2932. https://doi.org/10.3892/mmr.2015.3676