Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein

Hou,Yu  Sen; Liu,Ling  Ying; Chai,Jia  Ke; Yu,Yong  Hui; Duan,Hong  Jie; Hu,Quan; Yin,Hui  Nan; Wang,Yi  He; Zhuang,Shu  Bo; Fan,Jun; Chu,Wan  Li; Ma,Li

doi:10.3892/mmr.2015.3723

Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein

Authors:
- Yu Sen Hou
- Ling Ying Liu
- Jia Ke Chai
- Yong Hui Yu
- Hong Jie Duan
- Quan Hu
- Hui Nan Yin
- Yi He Wang
- Shu Bo Zhuang
- Jun Fan
- Wan Li Chu
- Li Ma
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Affiliations: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China
Published online on: May 4, 2015 https://doi.org/10.3892/mmr.2015.3723
Pages: 2521-2528
Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Mesenchymal stem cell (MSC)-based regenerative therapy is currently regarded as a novel approach with which to repair damaged tissues. However, the efficiency of MSC transplantation is limited due to the low survival rate of engrafted MSCs. Lipopolysaccharide (LPS) production is increased in numerous diseases and serves an essential function in the regulation of apoptosis in a variety of cell types. Previous studies have indicated that low‑dose LPS pretreatment contributes to cytoprotection. In the current study, LPS was demonstrated to induce apoptosis in human umbilical cord mesenchymal stem cells (hUCMSCs) via the activation of caspase, in a dose‑dependent manner. Low‑dose LPS pretreatment may protect hUCMSCs against apoptosis induced by high‑dose LPS, by upregulating the expression of cellular FADD‑like IL‑1β‑converting enzyme‑inhibitory protein (c‑FLIP). The results of the present study indicate that pretreatment with an appropriate concentration of LPS may alleviate high-dose LPS-induced apoptosis.

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