Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965

Qiao,Yuncheng; Guo,Dongming; Meng,Lei; Liu,Qingnan; Liu,Xiaohui; Tang,Chaoke; Yi,Guanghui; Wang,Zuo; Yin,Weidong; Tian,Guoping; Yuan,Zhonghua

doi:10.3892/mmr.2015.3864

Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells

Retraction in: /10.3892/mmr.2023.12965

Authors:
- Yuncheng Qiao
- Dongming Guo
- Lei Meng
- Qingnan Liu
- Xiaohui Liu
- Chaoke Tang
- Guanghui Yi
- Zuo Wang
- Weidong Yin
- Guoping Tian
- Zhonghua Yuan
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Affiliations: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Basic Nursing, Yiyang Medical College, Yiyang, Hunan 413000, P.R. China, Cyrus Tang Hematology Center (Research Partnership), Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215400, P.R. China, Department of Cardiovascular Medicine, The Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China
Published online on: May 27, 2015 https://doi.org/10.3892/mmr.2015.3864
Pages: 3599-3606

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Abstract

Oxidized low‑density lipoprotein (ox‑LDL) can increase the expression of adipophilin and the accumulation of intracellular lipid droplets. However, the detailed mechanisms remain to be fully elucidated. The present study aimed to investigate the mechanism underlying the effect of ox‑LDL on the expression of adipophilin and the accumulation of intracellular cholesterol esters. The results revealed that ox‑LDL increased the activation of protein kinase C α (PKCα), expression of adipophilin and acyl‑coenzymeA: cholesterol acyltransferse 1 (ACAT1) and increased accumulation of intracellular cholesterol esters. In addition, PKCα siRNA abrogated ox‑LDL‑induced adipophilin, expression of ATAC1 and accumulation of cholesterol esters. Furthermore, ox‑LDL increased the accumulation of intracellular cholesterol esters and expression of ACAT1, and this effect were reversed by transfection with adipophilin siRNA. Taken together, these results demonstrated that ox‑LDL induces the accumulation of cholesterol esters, which is mediated by the PKCα‑adipophilin‑ACAT1 pathway.

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