Helicobacter pylori cytotoxin‑associated gene A activates tumor necrosis factor‑α and interleukin‑6 in gastric epithelial cells through P300/CBP‑associated factor‑mediated nuclear factor‑κB p65 acetylation

  • Authors:
    • Qiong Lin
    • Hui Xu
    • Xintao Chen
    • Guorong Tang
    • Lan Gu
    • Yehong Wang
  • View Affiliations

  • Published online on: July 29, 2015     https://doi.org/10.3892/mmr.2015.4143
  • Pages: 6337-6345
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Abstract

Helicobacter pylori‑initiated chronic gastritis is characterized by the cytotoxin‑associated gene (Cag) pathogenicity island‑dependent upregulation of pro‑inflammatory cytokines in gastric epithelial cells, which is largely mediated by the activation of nuclear factor (NF)‑κB as a transcription factor. However, the precise regulation of NF‑κB activation, particularly post‑translational modifications in the CagA‑induced inflammatory response, has remained elusive. The present study showed that Helicobacter pylori CagA, an important virulence factor, induced the expression of P300/CBP‑associated factor (PCAF) in gastric epithelial cells. Further study revealed that PCAF was able to physically associate with the NF‑κB p65 sub‑unit and enhance its acetylation. More importantly, PCAF‑induced p65 acetylation was shown to contribute to p65 phosphorylation and further upregulation of tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 in gastric adenocarcinoma cells. In conclusion, the results of the present study indicated that Helicobacter pylori CagA enhanced TNF‑α and IL‑6 in gastric adenocarcinoma cells through PCAF‑mediated NF‑κB p65 sub‑unit acetylation.
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October-2015
Volume 12 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lin Q, Xu H, Chen X, Tang G, Gu L and Wang Y: Helicobacter pylori cytotoxin‑associated gene A activates tumor necrosis factor‑α and interleukin‑6 in gastric epithelial cells through P300/CBP‑associated factor‑mediated nuclear factor‑κB p65 acetylation. Mol Med Rep 12: 6337-6345, 2015.
APA
Lin, Q., Xu, H., Chen, X., Tang, G., Gu, L., & Wang, Y. (2015). Helicobacter pylori cytotoxin‑associated gene A activates tumor necrosis factor‑α and interleukin‑6 in gastric epithelial cells through P300/CBP‑associated factor‑mediated nuclear factor‑κB p65 acetylation. Molecular Medicine Reports, 12, 6337-6345. https://doi.org/10.3892/mmr.2015.4143
MLA
Lin, Q., Xu, H., Chen, X., Tang, G., Gu, L., Wang, Y."Helicobacter pylori cytotoxin‑associated gene A activates tumor necrosis factor‑α and interleukin‑6 in gastric epithelial cells through P300/CBP‑associated factor‑mediated nuclear factor‑κB p65 acetylation". Molecular Medicine Reports 12.4 (2015): 6337-6345.
Chicago
Lin, Q., Xu, H., Chen, X., Tang, G., Gu, L., Wang, Y."Helicobacter pylori cytotoxin‑associated gene A activates tumor necrosis factor‑α and interleukin‑6 in gastric epithelial cells through P300/CBP‑associated factor‑mediated nuclear factor‑κB p65 acetylation". Molecular Medicine Reports 12, no. 4 (2015): 6337-6345. https://doi.org/10.3892/mmr.2015.4143