Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes

  • Authors:
    • Tatiana Wojciechowicz
    • Marek Skrzypski
    • Paweł A. Kołodziejski
    • Dawid Szczepankiewicz
    • Ewa Pruszyńska‑Oszmałek
    • Przemysław Kaczmarek
    • Mathias Z. Strowski
    • Krzysztof W. Nowak
  • View Affiliations

  • Published online on: October 22, 2015     https://doi.org/10.3892/mmr.2015.4470
  • Pages: 8169-8175
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Abstract

Obestatin is a 23-amino acid peptide encoded by the ghrelin gene, which regulates food intake, body weight and insulin sensitivity. Obestatin influences glucose and lipid metabolism in mature adipocytes in rodents. However, the role of this peptide in rat preadipocytes remains to be fully understood. The current study characterized the effects of obestatin on lipid accumulation, preadipocyte differentiation, lipolysis and leptin secretion in rat primary preadipocytes. Obestatin enhanced lipid accumulation in rat preadipocytes and increased the expression of surrogate markers of preadipocyte differentiation. At the early stage of differentiation, obestatin suppressed lipolysis. By contrast, lipolysis was stimulated at the late stage of adipogenesis. Furthermore, obestatin stimulated the release of leptin, a key satiety hormone. Overall, the results indicated that obestatin promotes preadipocyte differentiation. Obestatin increased leptin release in preadipocytes, while the modulation of lipolysis appears to depend upon the stage of differentiation.
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December-2015
Volume 12 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wojciechowicz T, Skrzypski M, Kołodziejski PA, Szczepankiewicz D, Pruszyńska‑Oszmałek E, Kaczmarek P, Strowski MZ and Nowak KW: Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes. Mol Med Rep 12: 8169-8175, 2015.
APA
Wojciechowicz, T., Skrzypski, M., Kołodziejski, P.A., Szczepankiewicz, D., Pruszyńska‑Oszmałek, E., Kaczmarek, P. ... Nowak, K.W. (2015). Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes. Molecular Medicine Reports, 12, 8169-8175. https://doi.org/10.3892/mmr.2015.4470
MLA
Wojciechowicz, T., Skrzypski, M., Kołodziejski, P. A., Szczepankiewicz, D., Pruszyńska‑Oszmałek, E., Kaczmarek, P., Strowski, M. Z., Nowak, K. W."Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes". Molecular Medicine Reports 12.6 (2015): 8169-8175.
Chicago
Wojciechowicz, T., Skrzypski, M., Kołodziejski, P. A., Szczepankiewicz, D., Pruszyńska‑Oszmałek, E., Kaczmarek, P., Strowski, M. Z., Nowak, K. W."Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes". Molecular Medicine Reports 12, no. 6 (2015): 8169-8175. https://doi.org/10.3892/mmr.2015.4470