Open Access

Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells

Corrigendum in: /mmr/13/1/1054

  • Authors:
    • Dacai Xu
    • Jianglin Wang
    • Zhenkang Zhou
    • Zhiwei He
    • Qing Zhao
  • View Affiliations

  • Published online on: October 23, 2015     https://doi.org/10.3892/mmr.2015.4477
  • Pages: 7963-7970
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) is the leading cause of cancer-associated mortality worldwide; however, only limited therapeutic treatments are currently available. The present study aimed to investigate the effects of cannabinoids as novel therapeutic targets in HCC. In addition, the mechanism underlying the effects of a synthetic cannabinoid, WIN55, 212‑2, on the BEL7402 HCC cell line was investigated. The results demonstrated that WIN55, 212‑2 induced cell cycle arrest of the BEL7402 cells at the G0/G1 phase via cannabinoid receptor 2 (CB2)‑mediated downregulation of phosphorylated-extracellular signal-regulated kinases (ERK)1/2, upregulation of p27, and downregulation of cyclin D1 and cyclin‑dependent kinase 4. Furthermore, inhibition of CB2 with the CB2 antagonist AM630 abrogated WIN55, 212‑2‑induced cell cycle arrest. Inhibition of ERK1/2 also resulted in cell cycle dysregulation and cell cycle arrest at the G0/G1 phase, which subsequently resulted in cell growth inhibition. In addition, the present study detected a significant reduction in matrix metalloproteinase‑9, retinoblastoma protein and E2F1 expression, and migration inhibition by WIN treatment. These results suggested that cannabinoid receptor agonists, including WIN, may be considered as novel therapeutics for the treatment of HCC.
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December-2015
Volume 12 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xu D, Wang J, Zhou Z, He Z and Zhao Q: Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells Corrigendum in /mmr/13/1/1054. Mol Med Rep 12: 7963-7970, 2015.
APA
Xu, D., Wang, J., Zhou, Z., He, Z., & Zhao, Q. (2015). Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells Corrigendum in /mmr/13/1/1054. Molecular Medicine Reports, 12, 7963-7970. https://doi.org/10.3892/mmr.2015.4477
MLA
Xu, D., Wang, J., Zhou, Z., He, Z., Zhao, Q."Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells Corrigendum in /mmr/13/1/1054". Molecular Medicine Reports 12.6 (2015): 7963-7970.
Chicago
Xu, D., Wang, J., Zhou, Z., He, Z., Zhao, Q."Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells Corrigendum in /mmr/13/1/1054". Molecular Medicine Reports 12, no. 6 (2015): 7963-7970. https://doi.org/10.3892/mmr.2015.4477