MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma

  • Authors:
    • Xian Chen
    • Lianhua Bo
    • Wei Lu
    • Guihua Zhou
    • Qi Chen
  • View Affiliations

  • Published online on: November 2, 2015     https://doi.org/10.3892/mmr.2015.4500
  • Pages: 477-482
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Abstract

microRNA(miR)-148b has been found to be downregulated in various human malignancies, including hepatocellular carcinoma (HCC) as well as gastric, pancreatic, colon and oral cancer. However, the function of miR‑148b in HCC has remained elusive. The present study examined the effects of miR‑148b on the proliferation, migration and invasion of HCC cells in vitro. After transfection of the HepG2 and SMMC‑7721 HCC cell lines with miR‑148b, an MTT assay, a Transwell migration and invasion assay as well as western blot analysis were performed. miR-148b was shown to inhibit cell proliferation, migration and invasion in the two cell lines. Using a luciferase reporter assay, the present study also provided the first evidence that miR‑148b directly targets Rho‑associated protein kinase 1 in HCC. These results suggested that miR-148 may represent a novel molecular marker and a potential molecular therapeutic for inhibiting metastasis of HCC.
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January-2016
Volume 13 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chen X, Bo L, Lu W, Zhou G and Chen Q: MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma. Mol Med Rep 13: 477-482, 2016
APA
Chen, X., Bo, L., Lu, W., Zhou, G., & Chen, Q. (2016). MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma. Molecular Medicine Reports, 13, 477-482. https://doi.org/10.3892/mmr.2015.4500
MLA
Chen, X., Bo, L., Lu, W., Zhou, G., Chen, Q."MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma". Molecular Medicine Reports 13.1 (2016): 477-482.
Chicago
Chen, X., Bo, L., Lu, W., Zhou, G., Chen, Q."MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma". Molecular Medicine Reports 13, no. 1 (2016): 477-482. https://doi.org/10.3892/mmr.2015.4500