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Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells

  • Authors:
    • Yuan‑Fei Liu
    • Guo‑Qiang Qu
    • Yun‑Min Lu
    • Wu‑Ming Kong
    • Yuan Liu
    • Wei‑Xiong Chen
    • Xiao‑Hong Liao

  • View Affiliations

  • Published online on: November 6, 2015     https://doi.org/10.3892/mmr.2015.4510
  • Pages: 41-48

  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is the second most common cause of cancer‑associated mortality worldwide. Previous studies suggest that mitogen‑activated protein kinase kinase kinase kinase isoform 4 (MAP4K4) is involved in cancer cell growth, apoptosis and migration. In the present study, bioinformatics analysis and reverse transcription‑quantitative polymerase chain reaction were performed to determine if MAP4K4 was overexpressed in GC. The knockdown of MAP4K4 by RNA interference in GC cells markedly inhibited cell proliferation, which may be mediated by cell cycle arrest in the G1 phase. The silencing of MAP4K4 also induced cell apoptosis by increasing the ratio of Bax/Bcl‑2. In addition, Notch signaling was markedly reduced by MAP4K4 silencing. The results of the present study suggested that inhibition of MAP4K4 may be a therapeutic strategy for GC.
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January-2016
Volume 13 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Liu YF, Qu GQ, Lu YM, Kong WM, Liu Y, Chen WX and Liao XH: Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells. Mol Med Rep 13: 41-48, 2016.
APA
Liu, Y., Qu, G., Lu, Y., Kong, W., Liu, Y., Chen, W., & Liao, X. (2016). Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells. Molecular Medicine Reports, 13, 41-48. https://doi.org/10.3892/mmr.2015.4510
MLA
Liu, Y., Qu, G., Lu, Y., Kong, W., Liu, Y., Chen, W., Liao, X."Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells". Molecular Medicine Reports 13.1 (2016): 41-48.
Chicago
Liu, Y., Qu, G., Lu, Y., Kong, W., Liu, Y., Chen, W., Liao, X."Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells". Molecular Medicine Reports 13, no. 1 (2016): 41-48. https://doi.org/10.3892/mmr.2015.4510