Catalpol downregulates vascular endothelial‑cadherin expression and induces vascular hyperpermeability

Zhang,Caiqing; Liu,Qingfa; Dong,Fengyun; Li,Liqun; Du,Juan; Xie,Qi; Hu,Hesheng; Yan,Suhua; Zhou,Xia; Li,Changsheng; Lobe,Corrinne G.; Liu,Ju

doi:10.3892/mmr.2015.4522

Catalpol downregulates vascular endothelial‑cadherin expression and induces vascular hyperpermeability

Authors:
- Caiqing Zhang
- Qingfa Liu
- Fengyun Dong
- Liqun Li
- Juan Du
- Qi Xie
- Hesheng Hu
- Suhua Yan
- Xia Zhou
- Changsheng Li
- Corrinne G. Lobe
- Ju Liu
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Affiliations: Department of Respiratory Diseases, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Department of Traditional Chinese Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Miami Mice Research Corp., Toronto, ON M5G 1L7, Canada
Published online on: November 6, 2015 https://doi.org/10.3892/mmr.2015.4522
Pages: 373-378

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Abstract

Catalpol, an iridiod glucoside isolated from Rehmannia glutinosa, has been reported to possess anti‑inflammatory properties. However, the molecular mechanisms underlying this effect have not been fully elucidated. This study aimed to investigate the effects of catalpol on vascular permeability. Using Transwell permeability assays and measurements of trans‑endothelial electrical resistance (TEER), it was demonstrated that 1 mM catalpol induces a significant increase in the permeability of the monolayers of human umbilical vein endothelial cells (HUVECs). Western blotting and immunofluorescence demonstrated that catalpol inhibits the expression of vascular endothelial (VE)‑cadherin, the key component of adherens junctions, but not occludin, the major constituent of tight junctions. In addition, catalpol inhibits the ETS transcription factor ERG, a positive regulator of VE‑cadherin. Knockdown of ERG expression compromised the catalpol‑induced reduction of TEER in HUVECs. The present study revealed a novel effect of catalpol on vascular permeability and gave insight into the multifaceted roles of catalpol in inflammation.

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