Trichostatin A inhibits inflammation in phorbol myristate acetate‑induced macrophages by regulating the acetylation of histone and/or non‑histone proteins

  • Authors:
    • Qian Zhang
    • Fan Yang
    • Xun Li
    • Luwen Wang
    • Xiaogang Chu
    • Hong Zhang
    • Zuojiong Gong
  • View Affiliations

  • Published online on: November 20, 2015     https://doi.org/10.3892/mmr.2015.4594
  • Pages: 845-852
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Abstract

Histone deacetylase inhibitors (HDACi) are currently used in the routine clinical treatment of cancer. Alongside the antitumor activity of HDACi, increased attention has been paid to their anti‑inflammatory effects. The present study aimed to analyze the inhibitory effects of the HDACi Trichostatin A (TSA), on the release of inflammatory mediators from macrophages differentiated from U‑937 cells. A low dose of TSA (50 nM) was able to effectively decrease the levels of inflammatory cytokines in the cell supernatants, independent of apoptosis. In addition, the potential underlying mechanisms were explored, and TSA was shown to promote, rather than inhibit, the acetylation of histones. Furthermore, the inflammation‑induced enhanced expression of class I HDACs was effectively inhibited by TSA. In addition, TSA enhanced the lipopolysaccharide (LPS)‑induced expression of cyclooxygenase‑2, but suppressed the LPS‑induced expression of chemokine (C‑C motif) ligand 7. The acetylation level of nuclear factor‑κB p65 was decreased by LPS, but increased following treatment with TSA. In conclusion, TSA was able to inhibit inflammation in macrophages. However, whether the mechanism by which TSA inhibits inflammation is through significantly enhancing histone acetylation, in order to selectively suppress the expression of proinflammatory genes, and/or through regulating non‑histone acetylation requires further research.
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January-2016
Volume 13 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang Q, Yang F, Li X, Wang L, Chu X, Zhang H and Gong Z: Trichostatin A inhibits inflammation in phorbol myristate acetate‑induced macrophages by regulating the acetylation of histone and/or non‑histone proteins. Mol Med Rep 13: 845-852, 2016
APA
Zhang, Q., Yang, F., Li, X., Wang, L., Chu, X., Zhang, H., & Gong, Z. (2016). Trichostatin A inhibits inflammation in phorbol myristate acetate‑induced macrophages by regulating the acetylation of histone and/or non‑histone proteins. Molecular Medicine Reports, 13, 845-852. https://doi.org/10.3892/mmr.2015.4594
MLA
Zhang, Q., Yang, F., Li, X., Wang, L., Chu, X., Zhang, H., Gong, Z."Trichostatin A inhibits inflammation in phorbol myristate acetate‑induced macrophages by regulating the acetylation of histone and/or non‑histone proteins". Molecular Medicine Reports 13.1 (2016): 845-852.
Chicago
Zhang, Q., Yang, F., Li, X., Wang, L., Chu, X., Zhang, H., Gong, Z."Trichostatin A inhibits inflammation in phorbol myristate acetate‑induced macrophages by regulating the acetylation of histone and/or non‑histone proteins". Molecular Medicine Reports 13, no. 1 (2016): 845-852. https://doi.org/10.3892/mmr.2015.4594