Inhibitory effects of tetrandrine on epidermal growth factor-induced invasion and migration in HT29 human colorectal adenocarcinoma cells

  • Authors:
    • Chi‑Ting Horng
    • Jai‑Sing Yang
    • Jo‑Hua Chiang
    • Chi‑Cheng Lu
    • Chiu‑Fang Lee
    • Ni‑Na Chiang
    • Fu‑An Chen
  • View Affiliations

  • Published online on: December 1, 2015     https://doi.org/10.3892/mmr.2015.4635
  • Pages: 1003-1009
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Abstract

Tetrandrine has been shown to reduce cancer cell proliferation and to inhibit metastatic effects in multiple cancer models in vitro and in vivo. However, the effects of tetrandrine on the underlying mechanism of HT29 human colorectal adenocarcinoma cell metastasis remain to be fully elucidated. The aim of the present study was focused on tetrandrine‑treated HT29 cells following epidermal growth factor (EGF) treatment, and Transwell, gelatin zymography, gene expression and immunoblotting assays were performed to investigate metastatic effects in vitro. Tetrandrine was observed to dose‑dependently inhibit EGF‑induced HT29 cell invasion and migration, however, no effect on cell viability occurred following exposure to tetradrine between 0.5 and 2 µM. Tetrandrine treatment inhibited the enzymatic activity of matrix metalloprotease (MMP)‑2 and MMP‑9 in a concentration‑dependent manner. The present study also found a reduction in the mRNA expression levels of MMP‑2 and MMP‑9 in the tetrandrine‑treated HT29 cells. Tetrandrine also suppressed the phosphorylation of EGF receptor (EGFR) and its downstream pathway, including phosphoinositide‑dependent kinase 1, phosphatidylinositol 3‑kinase and phosphorylated AKT, suppressing the gene expression of MMP‑2 and MMP‑9. Furthermore, tetrandrine triggered mitogen‑activated protein kinase signaling through the suppressing the activation of phosphorylated extracellular signal‑regulated protein kinase. These data suggested that targeting EGFR signaling and its downstream molecules contributed to the inhibition of EGF‑induced HT29 cell metastasis caused by tetrandrine, eventually leading to a reduction in the mRNA and gelatinase activities of MMP-2 and MMP-9, respectively.
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January-2016
Volume 13 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Horng CT, Yang JS, Chiang JH, Lu CC, Lee CF, Chiang NN and Chen FA: Inhibitory effects of tetrandrine on epidermal growth factor-induced invasion and migration in HT29 human colorectal adenocarcinoma cells. Mol Med Rep 13: 1003-1009, 2016
APA
Horng, C., Yang, J., Chiang, J., Lu, C., Lee, C., Chiang, N., & Chen, F. (2016). Inhibitory effects of tetrandrine on epidermal growth factor-induced invasion and migration in HT29 human colorectal adenocarcinoma cells. Molecular Medicine Reports, 13, 1003-1009. https://doi.org/10.3892/mmr.2015.4635
MLA
Horng, C., Yang, J., Chiang, J., Lu, C., Lee, C., Chiang, N., Chen, F."Inhibitory effects of tetrandrine on epidermal growth factor-induced invasion and migration in HT29 human colorectal adenocarcinoma cells". Molecular Medicine Reports 13.1 (2016): 1003-1009.
Chicago
Horng, C., Yang, J., Chiang, J., Lu, C., Lee, C., Chiang, N., Chen, F."Inhibitory effects of tetrandrine on epidermal growth factor-induced invasion and migration in HT29 human colorectal adenocarcinoma cells". Molecular Medicine Reports 13, no. 1 (2016): 1003-1009. https://doi.org/10.3892/mmr.2015.4635