Open Access

Dendritic cells pulsed with Hsp70 and HBxAg induce specific antitumor immune responses in hepatitis B virus‑associated hepatocellular carcinoma

  • Authors:
    • Hui Wang
    • Fang Feng
    • Xiao‑Ping Wang
    • Rou‑Shu Wang
    • Ying Wu
    • Min‑Gao Zhu
    • Hong Zhang
    • Zhi‑Xiang Zhuang
  • View Affiliations

  • Published online on: December 7, 2015     https://doi.org/10.3892/mmr.2015.4654
  • Pages: 1077-1082
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have drawn attention to dendritic cell (DC) vaccines; particularly the application of the tumor‑associated antigen‑targeted DC vaccine. The present study analyzed DCs derived from a normal individual and pulsed the cells with heat shock protein 70 peptide (Hsp70) and/or hepatitis B virus x antigen (HBxAg), a hepatocellular carcinoma (HCC)‑associated antigen. It was then investigated whether this method of vaccination induced strong therapeutic antitumor immunity. The results revealed that the Hsp70/HBxAg complex‑activated phenotype improves the functional maturation of DCs compared with using Hsp70 or HBxAg alone. Compared with either Hsp70 or HBxAg alone, matured DCs pulsed with the Hsp70/HBxAg complex stimulated a high level of autologous T‑cell proliferation and induced HCC‑specific cytotoxic T lymphocytes, which specifically killed HCC cells through a major histocompatibility complex class I mechanism. These results indicated that a vaccination therapy using DCs co‑pulsed with the Hsp70/HBxAg complex is an effective strategy for immunotherapy and may offer a useful approach to protect against HCC.
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February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang H, Feng F, Wang XP, Wang RS, Wu Y, Zhu MG, Zhang H and Zhuang ZX: Dendritic cells pulsed with Hsp70 and HBxAg induce specific antitumor immune responses in hepatitis B virus‑associated hepatocellular carcinoma. Mol Med Rep 13: 1077-1082, 2016.
APA
Wang, H., Feng, F., Wang, X., Wang, R., Wu, Y., Zhu, M. ... Zhuang, Z. (2016). Dendritic cells pulsed with Hsp70 and HBxAg induce specific antitumor immune responses in hepatitis B virus‑associated hepatocellular carcinoma. Molecular Medicine Reports, 13, 1077-1082. https://doi.org/10.3892/mmr.2015.4654
MLA
Wang, H., Feng, F., Wang, X., Wang, R., Wu, Y., Zhu, M., Zhang, H., Zhuang, Z."Dendritic cells pulsed with Hsp70 and HBxAg induce specific antitumor immune responses in hepatitis B virus‑associated hepatocellular carcinoma". Molecular Medicine Reports 13.2 (2016): 1077-1082.
Chicago
Wang, H., Feng, F., Wang, X., Wang, R., Wu, Y., Zhu, M., Zhang, H., Zhuang, Z."Dendritic cells pulsed with Hsp70 and HBxAg induce specific antitumor immune responses in hepatitis B virus‑associated hepatocellular carcinoma". Molecular Medicine Reports 13, no. 2 (2016): 1077-1082. https://doi.org/10.3892/mmr.2015.4654