Effect of bone marrow-derived stem cells on chondrocytes from patients with osteoarthritis

Zhang,Qiangzhi; Chen,Yong; Wang,Qiang; Fang,Chaoyong; Sun,Yu; Yuan,Tao; Wang,Yuebei; Bao,Rongni; Zhao,Ningjian

doi:10.3892/mmr.2015.4720

Effect of bone marrow-derived stem cells on chondrocytes from patients with osteoarthritis

Authors:
- Qiangzhi Zhang
- Yong Chen
- Qiang Wang
- Chaoyong Fang
- Yu Sun
- Tao Yuan
- Yuebei Wang
- Rongni Bao
- Ningjian Zhao
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Affiliations: Department of Orthopedics, Subei People's Hospital of Jiangsu, Yangzhou, Jiangsu 225001, P.R. China, Department of Oncology, Subei People's Hospital of Jiangsu, Yangzhou, Jiangsu 225001, P.R. China, Department of Orthopedics, Jinling Hospital, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China
Published online on: December 28, 2015 https://doi.org/10.3892/mmr.2015.4720
Pages: 1795-1800

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Abstract

Increasing numbers of individuals are suffering from osteoarthritis every year, and the directed intra‑articular injection of bone marrow stem cells has provided a promising treatment strategy for osteoarthritis. Although a number of studies have demonstrated that intra‑articular injection of bone marrow stem cells produced desirable results, the mechanism underlying this effect has not been elucidated. In the current study, the effect of bone marrow stem cells on chondrocytes from patients with osteoarthritis was observed in a co‑culture system. Human chondrocytes were obtained from patients with osteoarthritis who underwent surgical procedures and bone marrow stem cells were obtained from bone marrow aspirates, and then the chondrocytes were then cultured alone or cocultured with bone marrow stem cells in 0.4-µm Transwell inserts. The differentiation and biological activity of chondrocytes in the culture system were measured, and the inflammatory factors and OA‑associated markers were also measured. The results indicated that coculture with human bone marrow stem cells increases cell proliferation of chondrocytes and inhibits inflammatory activity in osteoarthritis.

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