Downregulation of fatty acid synthase complex suppresses cell migration by targeting phospho‑AKT in bladder cancer

  • Authors:
    • Shuai‑Shuai Zheng
    • Jian‑Gang Gao
    • Zhi‑Jun Liu
    • Xin‑Hong Zhang
    • Shuai Wu
    • Bo‑Wen Weng
    • You‑Lin Wang
    • Si‑Chuan Hou
    • Bo Jiang
  • View Affiliations

  • Published online on: December 30, 2015     https://doi.org/10.3892/mmr.2015.4746
  • Pages: 1845-1850
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Abstract

The aim of the present study was to investigate the effect of fatty acid synthase complex (FASN) on the migration capacity of bladder transitional cell carcinoma (BTCC) cells and the involvement of matrix metalloproteinase‑9 (MMP‑9) via targeting of phospho‑AKT (p‑AKT). FASN‑specific small‑interfering RNA (FASN‑siRNA) was used to inhibit FASN gene expression in the 5637 and 253J BTCC cell lines. The knockdown efficiency of FAM‑conjugated FASN‑siRNA was confirmed by fluorescence microscopy. The migratory abilities of BTCC cells were assessed using a Transwell assay. Furthermore, protein and mRNA expression of FASN, p‑AKT, AKT, and migration‑associated protein MMP‑9 were detected by western blot analysis. Treatment with FASN inhibitor Cer and FASN‑siRNA decreased the migratory capacity of bladder cancer cells and reduced the levels of p‑AKT as well as the expression of MMP‑9. These results indicated that FASN inhibition suppressed the migratory capacity of BTCC cells through suppressing AKT activation and consequently reducing MMP‑9 expression. Targeting FASN may represent a promising novel therapeutic strategy for BTCC.
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February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zheng SS, Gao JG, Liu ZJ, Zhang XH, Wu S, Weng BW, Wang YL, Hou SC and Jiang B: Downregulation of fatty acid synthase complex suppresses cell migration by targeting phospho‑AKT in bladder cancer. Mol Med Rep 13: 1845-1850, 2016.
APA
Zheng, S., Gao, J., Liu, Z., Zhang, X., Wu, S., Weng, B. ... Jiang, B. (2016). Downregulation of fatty acid synthase complex suppresses cell migration by targeting phospho‑AKT in bladder cancer. Molecular Medicine Reports, 13, 1845-1850. https://doi.org/10.3892/mmr.2015.4746
MLA
Zheng, S., Gao, J., Liu, Z., Zhang, X., Wu, S., Weng, B., Wang, Y., Hou, S., Jiang, B."Downregulation of fatty acid synthase complex suppresses cell migration by targeting phospho‑AKT in bladder cancer". Molecular Medicine Reports 13.2 (2016): 1845-1850.
Chicago
Zheng, S., Gao, J., Liu, Z., Zhang, X., Wu, S., Weng, B., Wang, Y., Hou, S., Jiang, B."Downregulation of fatty acid synthase complex suppresses cell migration by targeting phospho‑AKT in bladder cancer". Molecular Medicine Reports 13, no. 2 (2016): 1845-1850. https://doi.org/10.3892/mmr.2015.4746