Native low density lipoprotein promotes lipid raft formation in macrophages

Song,Jian; Ping,Ling‑Yan; Duong,Duc M.; Gao,Xiao‑Yan; He,Chun‑Yan; Wei,Lei; Wu,Jun‑Zhu

doi:10.3892/mmr.2016.4781

Native low density lipoprotein promotes lipid raft formation in macrophages

Authors:
- Jian Song
- Ling‑Yan Ping
- Duc M. Duong
- Xiao‑Yan Gao
- Chun‑Yan He
- Lei Wei
- Jun‑Zhu Wu
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Affiliations: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA, Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: January 14, 2016 https://doi.org/10.3892/mmr.2016.4781
Pages: 2087-2093
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oxidized low‑density lipoprotein (LDL) has an important role in atherogenesis; however, the mechanisms underlying cell‑mediated LDL oxidation remain to be elucidated. The present study investigated whether native‑LDL induced lipid raft formation, in order to gain further insight into LDL oxidation. Confocal microscopic analysis revealed that lipid rafts were aggregated or clustered in the membrane, which were colocalized with myeloperoxidase (MPO) upon native LDL stimulation; however, in the presence of methyl‑β‑cyclodextrin (MβCD), LDL‑stimulated aggregation, translocation, and colocalization of lipid rafts components was abolished.. In addition, lipid raft disruptors MβCD and filipin decreased malondialdehyde expression levels. Density gradient centrifugation coupled to label‑free quantitative proteomic analysis identified 1,449 individual proteins, of which 203 were significantly upregulated following native‑LDL stimulation. Functional classification of the proteins identified in the lipid rafts revealed that the expression levels of translocation proteins were upregulated. In conclusion, the results of the present study indicated that native‑LDL induced lipid raft clustering in macrophages, and the expression levels of several proteins were altered in the stimulated macrophages, which provided novel insights into the mechanism underlying LDL oxidation.

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