CXCR4 antagonist AMD3100 ameliorates thyroid damage in autoimmune thyroiditis in NOD.H‑2h4 mice

  • Authors:
    • Xin Liu
    • Jinyuan Mao
    • Cheng Han
    • Shiqiao Peng
    • Chenyan Li
    • Ting Jin
    • Chenling Fan
    • Zhongyan Shan
    • Weiping Teng
  • View Affiliations

  • Published online on: March 2, 2016     https://doi.org/10.3892/mmr.2016.4965
  • Pages: 3604-3612
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Abstract

CXC chemokine ligand 12 (CXCL12) and its receptor, CXC chemokine receptor 4 (CXCR4), are upregulated in mice with autoimmune thyroid diseases. However, whether this interaction is involved in the pathophysiology of autoimmune thyroiditis (AIT) remains to be elucidated. In the present study, the effects of the CXCR4 antagonist, AMD3100, in an iodine‑induced autoimmune thyroiditis model were investigated. NOD.H‑2h4 mice were randomly separated into a control, AIT and AIT+AMD3100 groups. The mice were fed with 0.05% sodium iodide water for 8 weeks to induce AIT. The AMD3100‑treated mice were administered with the CXCR4 antagonist at a dose of 10 mg/kg intraperitoneally three times a week during the experimental period. The percentages of CD19+interleukin (IL)10+ B cells and CD4+IL10+ T cells, and the mRNA expression levels of IL10 in the splenocytes were reduced in the AIT group, compared with the control group, however, they increased following AMD3100 treatment, compared with the untreated AIT group. The percentages of CD4+ T cells, CD8+ T cells, CD19+ B cells and CD8+ interferon (IFN)γ+ T cells, and the mRNA expression levels of IFNγ increased in the AIT group, compared with the control group, however, these were reduced in the AMD3100 group, compared with the AIT group. The AMD3100‑treated mice also had lower serum thyroglobulin antibody titers and reduced lymphocytic infiltration in the thyroid, compared with the untreated AIT mice. These results suggested that inhibition of this chemokine axis may offer potential as a therapeutic target for the treatment of AIT.
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April-2016
Volume 13 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu X, Mao J, Han C, Peng S, Li C, Jin T, Fan C, Shan Z and Teng W: CXCR4 antagonist AMD3100 ameliorates thyroid damage in autoimmune thyroiditis in NOD.H‑2h4 mice. Mol Med Rep 13: 3604-3612, 2016.
APA
Liu, X., Mao, J., Han, C., Peng, S., Li, C., Jin, T. ... Teng, W. (2016). CXCR4 antagonist AMD3100 ameliorates thyroid damage in autoimmune thyroiditis in NOD.H‑2h4 mice. Molecular Medicine Reports, 13, 3604-3612. https://doi.org/10.3892/mmr.2016.4965
MLA
Liu, X., Mao, J., Han, C., Peng, S., Li, C., Jin, T., Fan, C., Shan, Z., Teng, W."CXCR4 antagonist AMD3100 ameliorates thyroid damage in autoimmune thyroiditis in NOD.H‑2h4 mice". Molecular Medicine Reports 13.4 (2016): 3604-3612.
Chicago
Liu, X., Mao, J., Han, C., Peng, S., Li, C., Jin, T., Fan, C., Shan, Z., Teng, W."CXCR4 antagonist AMD3100 ameliorates thyroid damage in autoimmune thyroiditis in NOD.H‑2h4 mice". Molecular Medicine Reports 13, no. 4 (2016): 3604-3612. https://doi.org/10.3892/mmr.2016.4965