Role of extracellular signal‑regulated kinase 1/2 signal transduction pathway in insulin secretion by β‑TC6 cells

Niu,Ben; Liu,Lijuan; Su,Heng; Xia,Xueshan; He,Qiu; Feng,Yue; Xue,Yuanming; Yan,Xinming

doi:10.3892/mmr.2016.5053

Role of extracellular signal‑regulated kinase 1/2 signal transduction pathway in insulin secretion by β‑TC6 cells

Authors:
- Ben Niu
- Lijuan Liu
- Heng Su
- Xueshan Xia
- Qiu He
- Yue Feng
- Yuanming Xue
- Xinming Yan
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Affiliations: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China, Department of Cadre Ward, WISCO General Hospital, Wuhan, Hubei 430080, P.R. China, Department of Endocrinology, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan, Kunming, Yunnan 650032, P.R. China, Institute of Basic and Clinical Medicine, Center of Clinical Molecular Biology of Yunnan, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan, Kunming, Yunnan 650032, P.R. China
Published online on: March 28, 2016 https://doi.org/10.3892/mmr.2016.5053
Pages: 4451-4454

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Abstract

The present study aimed to investigate the role of the extracellular signal-regulated kinase (ERK)1/2 signal transduction pathway in glucose‑stimulated insulin secretion in β‑TC6 mouse pancreatic cells. Insulin production by β‑TC6 cells was stimulated with various concentrations of glucose, which was dose-dependently inhibited by mitogen‑activated protein kinase inhibitor PD98059, as indicated by a radioimmunoassay. Furthermore, glucose stimulation enhanced the phosphorylation of ERK1/2, which was dose-dependently inhibited by PD98059, as indicated by western blot analysis. These results indicated that the activation of the ERK1/2 signal transduction pathway may have an important role in glucose‑stimulated insulin secretion in β‑TC6 cells.

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