shRNA-mediated RPS15A silencing inhibits U937 acute myeloid leukemia cell proliferation and enhances apoptosis

  • Authors:
    • Guangyao Li
    • Li Zhang
    • Jizhu Liu
    • Taiwu Xiao
    • Guozhen Liu
    • Jingxia Wang
    • Ming Hou
  • View Affiliations

  • Published online on: March 30, 2016     https://doi.org/10.3892/mmr.2016.5064
  • Pages: 4400-4406
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Abstract

Ribosomal protein S15a (RPS15A), which is a component of the 40S ribosomal subunit, is able to promote mRNA/ribosome interaction during the early stage of translation. Previous studies have demonstrated that RPS15A regulates cell growth and is involved in several types of human cancer. The aim of the present study was to investigate the role of RPS15A in acute myeloid leukemia (AML). Lentivirus‑delivered short hairpin RNA (shRNA) was used to silence RPS15A expression in the U937 AML cell line. Subsequently, the effects of RPS15A silencing on cell viability, cell cycle progression and apoptosis were investigated. The results indicated that RPS15A knockdown significantly inhibited cell growth. Furthermore, flow cytometric analysis demonstrated that the majority of U937 cells were arrested in G0/G1 phase and sub‑G1 phase after RPS15A knockdown, as determined using propidium iodide staining. In addition, U937 cells underwent apoptosis in response to RPS15A silencing, as determined using Annexin V/7‑aminoactinomycin D staining. In conclusion, the present study provides novel evidence indicating that RPS15A modulates AML cell growth in vitro, and may be considered a novel therapeutic target for the treatment of AML.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li G, Zhang L, Liu J, Xiao T, Liu G, Wang J and Hou M: shRNA-mediated RPS15A silencing inhibits U937 acute myeloid leukemia cell proliferation and enhances apoptosis. Mol Med Rep 13: 4400-4406, 2016.
APA
Li, G., Zhang, L., Liu, J., Xiao, T., Liu, G., Wang, J., & Hou, M. (2016). shRNA-mediated RPS15A silencing inhibits U937 acute myeloid leukemia cell proliferation and enhances apoptosis. Molecular Medicine Reports, 13, 4400-4406. https://doi.org/10.3892/mmr.2016.5064
MLA
Li, G., Zhang, L., Liu, J., Xiao, T., Liu, G., Wang, J., Hou, M."shRNA-mediated RPS15A silencing inhibits U937 acute myeloid leukemia cell proliferation and enhances apoptosis". Molecular Medicine Reports 13.5 (2016): 4400-4406.
Chicago
Li, G., Zhang, L., Liu, J., Xiao, T., Liu, G., Wang, J., Hou, M."shRNA-mediated RPS15A silencing inhibits U937 acute myeloid leukemia cell proliferation and enhances apoptosis". Molecular Medicine Reports 13, no. 5 (2016): 4400-4406. https://doi.org/10.3892/mmr.2016.5064