Open Access

Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn

  • Authors:
    • Fang Li
    • You‑Bo Zhang
    • Xia Wei
    • Chun‑Hong Song
    • Ming‑Qi Qiao
    • Hui‑Yun Zhang
  • View Affiliations

  • Published online on: April 4, 2016     https://doi.org/10.3892/mmr.2016.5082
  • Pages: 4191-4204
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The Chinese herbal formula, Shu-Yu capsule (SYC), has been successfully used to treat depression-like disorders in clinical settings. To rapidly identify the chemical constituents of SYC and its metabolites in rat serum, a simple and sensitive liquid chromatography-tandem mass spectrometry method was established in the present study. By comparing the retention times, MS and MSn spectra data in the literature and reference standards, a total of 73 compounds were identified from SYC. In rat serum, 62 components, including 13 prototype compounds and 49 metabolites were identified. Of these components, 14 metabolites were confirmed as novel metabolites of SYC. The results of the present study indicated that certain flavonoid glycosides and monoterpene glycosides were absorbed directly. Glucuronidation and sulfation were identified as the predominant metabolic pathways of the components in SYC. In addition, certain phase I reactions, including hydrolysis, demethylation and hydroxylation occurred in the rats. These results provide scientific evidence, which support further investigations of the pharmacology and mechanism of SYC.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li F, Zhang YB, Wei X, Song CH, Qiao MQ and Zhang HY: Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn. Mol Med Rep 13: 4191-4204, 2016.
APA
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., & Zhang, H. (2016). Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn. Molecular Medicine Reports, 13, 4191-4204. https://doi.org/10.3892/mmr.2016.5082
MLA
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., Zhang, H."Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn". Molecular Medicine Reports 13.5 (2016): 4191-4204.
Chicago
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., Zhang, H."Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn". Molecular Medicine Reports 13, no. 5 (2016): 4191-4204. https://doi.org/10.3892/mmr.2016.5082