Anti‑inflammatory and antioxidant activity of the traditional herbal formula Gwakhyangjeonggi‑san via enhancement of heme oxygenase‑1 expression in RAW264.7 macrophages

  • Authors:
    • Soo‑Jin Jeong
    • Ohn‑Soon Kim
    • Sae‑Rom Yoo
    • Chang‑Seob Seo
    • Yeji Kim
    • Hyeun‑Kyoo Shin
  • View Affiliations

  • Published online on: April 4, 2016     https://doi.org/10.3892/mmr.2016.5084
  • Pages: 4365-4371
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Abstract

Gwakhyangjeonggi‑san (GHJGS) is a mixture of herbal plants, including Agastache rugosa, Perilla frutescens, Angelica dahurica, Areca catechu, Poria cocos, Magnolia officinalis, Atractylodes macrocephala, Citrus reticulata, Pinellia ternata, Platycodon grandiflorum, Glycyrrhiza uralensis, Ziziphus jujuba and Zingiber officinale. GHJGS has been used for treating diarrhea‑predominant irritable bowel syndrome in traditional Korean medicine. In the present study, the anti‑inflammatory and antioxidant effects of GHJGS were investigated using the RAW 264.7 murine macrophage cell line. GHJGS significantly reduced production of the proinflammatory cytokines, tumor necrosis factor‑α, interleukin‑6 and prostaglandin E2 in lipopolysaccharide (LPS)‑stimulated macrophages. GHJGS markedly suppressed LPS‑induced phosphorylation of mitogen‑activated protein kinases, whereas it had no effect on nuclear factor‑κB activation. Furthermore, GHJGS enhanced expression of heme oxygenase‑1 and prevented the generation of reactive oxygen species in RAW 264.7 cells. These results indicate that GHJGS is a viable therapeutic agent against inflammation and oxidative stress‑associated disorders.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Jeong SJ, Kim OS, Yoo SR, Seo CS, Kim Y and Shin HK: Anti‑inflammatory and antioxidant activity of the traditional herbal formula Gwakhyangjeonggi‑san via enhancement of heme oxygenase‑1 expression in RAW264.7 macrophages. Mol Med Rep 13: 4365-4371, 2016.
APA
Jeong, S., Kim, O., Yoo, S., Seo, C., Kim, Y., & Shin, H. (2016). Anti‑inflammatory and antioxidant activity of the traditional herbal formula Gwakhyangjeonggi‑san via enhancement of heme oxygenase‑1 expression in RAW264.7 macrophages. Molecular Medicine Reports, 13, 4365-4371. https://doi.org/10.3892/mmr.2016.5084
MLA
Jeong, S., Kim, O., Yoo, S., Seo, C., Kim, Y., Shin, H."Anti‑inflammatory and antioxidant activity of the traditional herbal formula Gwakhyangjeonggi‑san via enhancement of heme oxygenase‑1 expression in RAW264.7 macrophages". Molecular Medicine Reports 13.5 (2016): 4365-4371.
Chicago
Jeong, S., Kim, O., Yoo, S., Seo, C., Kim, Y., Shin, H."Anti‑inflammatory and antioxidant activity of the traditional herbal formula Gwakhyangjeonggi‑san via enhancement of heme oxygenase‑1 expression in RAW264.7 macrophages". Molecular Medicine Reports 13, no. 5 (2016): 4365-4371. https://doi.org/10.3892/mmr.2016.5084