Open Access

Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats

  • Authors:
    • Lianqun Jia
    • Nan Song
    • Guanlin Yang
    • Yixin Ma
    • Xuetao Li
    • Ren Lu
    • Huimin Cao
    • Ni Zhang
    • Meilin Zhu
    • Junyan Wang
    • Xue Leng
    • Yuan Cao
    • Ying Du
    • Yue Xu
  • View Affiliations

  • Published online on: April 14, 2016     https://doi.org/10.3892/mmr.2016.5133
  • Pages: 4627-4635
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tanshinone IIA is the active compound isolated from Salvia miltiorrhiza bunge, which is a traditional Chinese medicine known as Danshen. The aim of the present study was to assess the effect of Tanshinone IIA on the regulation of lipid metabolism in the livers of hyperlipidemic rats and the underlying molecular events. An in vivo model of hyperlipidemia was established in rats, with the animals receiving a daily dose of Tanshinone IIA. The serum lipid profiles were analyzed using an automatic biochemical analyzer, and the histopathological alterations and lipid deposition in liver tissue were assessed using hematoxylin and eosin staining, and oil red O staining, respectively. The mRNA expression levels of microRNA (miR)‑33a, ATP‑binding cassette transporter (ABC)A1, ABCG1, sterol regulatory element‑binding protein 2 (SREBP‑2), proprotein convertase subtilisin/kexin type 9 (Pcsk9) and low‑density lipoprotein receptor (LDL‑R) in liver tissues were measured using reverse transcription‑quantitative polymerase chain reaction, and the protein expression levels of ABCA1, ABCG1, SREBP‑2, Pcsk9, and LDL‑R were analyzed using western blotting. Tanshinone IIA reduced lipid deposition and improved histopathology in the rat liver tissue, however, did not alter the lipid profile in rat serum. In addition, Tanshinone IIA treatment suppressed the expression of miR‑33a, whereas the protein expression levels of ABCA1, SREBP‑2, Pcsk9 in addition to LDL‑R mRNA and protein were upregulated. In conclusion, the present study indicated that Tanshinone IIA attenuated lipid deposition in the livers of hyperlipidemic rats and modulated the expression of miR‑33a and SREBP‑2/Pcsk9 signaling pathway proteins.
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June-2016
Volume 13 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Jia L, Song N, Yang G, Ma Y, Li X, Lu R, Cao H, Zhang N, Zhu M, Wang J, Wang J, et al: Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats. Mol Med Rep 13: 4627-4635, 2016
APA
Jia, L., Song, N., Yang, G., Ma, Y., Li, X., Lu, R. ... Xu, Y. (2016). Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats. Molecular Medicine Reports, 13, 4627-4635. https://doi.org/10.3892/mmr.2016.5133
MLA
Jia, L., Song, N., Yang, G., Ma, Y., Li, X., Lu, R., Cao, H., Zhang, N., Zhu, M., Wang, J., Leng, X., Cao, Y., Du, Y., Xu, Y."Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats". Molecular Medicine Reports 13.6 (2016): 4627-4635.
Chicago
Jia, L., Song, N., Yang, G., Ma, Y., Li, X., Lu, R., Cao, H., Zhang, N., Zhu, M., Wang, J., Leng, X., Cao, Y., Du, Y., Xu, Y."Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats". Molecular Medicine Reports 13, no. 6 (2016): 4627-4635. https://doi.org/10.3892/mmr.2016.5133