CD4+ T cell responses in Balb/c mice with food allergy induced by trinitrobenzene sulfonic acid and ovalbumin

Sun,Chen‑Yi; Bai,Jie; Hu,Tian‑Yong; Cheng,Bao‑Hui; Ma,Li; Fan,Xiao‑Qin; Yang,Ping‑Chang; Zheng,Peng‑Yuan; Liu,Zhi‑Qiang

doi:10.3892/mmr.2016.5153

CD4+ T cell responses in Balb/c mice with food allergy induced by trinitrobenzene sulfonic acid and ovalbumin

Authors:
- Chen‑Yi Sun
- Jie Bai
- Tian‑Yong Hu
- Bao‑Hui Cheng
- Li Ma
- Xiao‑Qin Fan
- Ping‑Chang Yang
- Peng‑Yuan Zheng
- Zhi‑Qiang Liu
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Affiliations: Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Maternal and Child Care Service Centre of Zhengzhou, Zhengzhou, Henan 471000, P.R. China, Shenzhen Key Laboratory of Ear, Nose and Throat (ENT), Department of Rhinology, Institute of ENT, Longgang ENT Hospital, Shenzhen, Guangdong 518116, P.R. China
Published online on: April 21, 2016 https://doi.org/10.3892/mmr.2016.5153
Pages: 5349-5357

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Abstract

The rapid increase in atopic diseases is potentially linked to increased hapten exposure, however, the role of haptens in the pathogenesis of food allergy remains unknown. Further studies are required to elucidate the cluster of differentiation 4 positive (CD4+) T cell response to food allergy induced by haptens. Dendritic cells were primed by trinitrobenzene sulfonic acid (TNBS) as a hapten or ovalbumin (OVA) as a model antigen, in a cell culture model. BALB/c mice were sensitized using TNBS and/or OVA. Intestinal Th1/Th2 cell and ovalbumin specific CD4+ T cells proliferation, intestinal cytokines (interleukin‑4 and interferon‑γ) in CD4+ T cells were evaluated. TNBS increased the expression of T cell immunoglobulin and mucin domain‑4 and tumor necrosis factor ligand superfamily member 4 in dendritic cells. Skewed Th2 cell polarization, extensive expression of interleukin‑4, reduced expression of interferon‑γ and forkhead box protein P3 were elicited following concomitant exposure to TNBS and OVA, with reduced regulatory T cells in the mouse intestinal mucosa, whereas a Th1 response was detected when challenged by TNBS or OVA alone. This data suggests that TNBS, as a hapten, combined with food antigens may lead to a Th2 cell response in the intestinal mucosa.

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