TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma

  • Authors:
    • Jinqian Liang
    • Dan Xing
    • Zheng Li
    • Jianxiong Shen
    • Hong Zhao
    • Shugang Li
  • View Affiliations

  • Published online on: April 25, 2016     https://doi.org/10.3892/mmr.2016.5183
  • Pages: 5200-5206
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Abstract

Osteosarcoma is a prevalent type of cancer and has a high metastatic ability, particularly for metastasis to the lungs. Effective treatment strategies have improved, however, the detailed molecular mechanism underlying the onset of this malignancy remains to be fully elucidated. The current study investigated the role of the tripartite motif (TRIM) family protein TRIM59 in osteosarcoma growth and metastasis. It was identified that TRIM59 was overexpressed in clinical osteosarcoma tissues and cultured osteosarcoma cell lines. In addition, the MTT assay demonstrated that in U2OS and MG63 cells, knockdown of TRIM59 by specific siRNA inhibited proliferation, whereas overexpression of TRIM59 promoted cell proliferation. Furthermore, overexpression of TRIM59 significantly increased the U2OS cell migrative and invasive abilities in a Transwell chamber assay. In addition, TRIM59 was able to negatively regulate the protein levels of P53 without significantly affecting the mRNA levels in U2OS and MG63 cells. These data suggest the oncogenic abilities of TRIM59 in osteosarcoma, which promote osteosarcoma cell proliferation, migration and invasion.
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June-2016
Volume 13 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liang J, Xing D, Li Z, Shen J, Zhao H and Li S: TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma. Mol Med Rep 13: 5200-5206, 2016.
APA
Liang, J., Xing, D., Li, Z., Shen, J., Zhao, H., & Li, S. (2016). TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma. Molecular Medicine Reports, 13, 5200-5206. https://doi.org/10.3892/mmr.2016.5183
MLA
Liang, J., Xing, D., Li, Z., Shen, J., Zhao, H., Li, S."TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma". Molecular Medicine Reports 13.6 (2016): 5200-5206.
Chicago
Liang, J., Xing, D., Li, Z., Shen, J., Zhao, H., Li, S."TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma". Molecular Medicine Reports 13, no. 6 (2016): 5200-5206. https://doi.org/10.3892/mmr.2016.5183